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Reference: Grant-Downton, R, Le Trionnaire, G, Schmid, R et al., (2009). MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana. BMC genomics, 10 (1), Article no. 643.Citable link to this page:

 

MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana.

Abstract: BACKGROUND: New generation sequencing technology has allowed investigation of the small RNA populations of flowering plants at great depth. However, little is known about small RNAs in their reproductive cells, especially in post-meiotic cells of the gametophyte generation. Pollen - the male gametophyte - is the specialised haploid structure that generates and delivers the sperm cells to the female gametes at fertilisation. Whether development and differentiation of the male gametophyte depends on the action of microRNAs and trans-acting siRNAs guiding changes in gene expression is largely unknown. Here we have used 454 sequencing to survey the various small RNA populations present in mature pollen of Arabidopsis thaliana. RESULTS: In this study we detected the presence of 33 different microRNA families in mature pollen and validated the expression levels of 17 selected miRNAs by Q-RT-PCR. The majority of the selected miRNAs showed pollen-enriched expression compared with leaves. Furthermore, we report for the first time the presence of trans-acting siRNAs in pollen. In addition to describing new patterns of expression for known small RNAs in each of these classes, we identified 7 putative novel microRNAs. One of these, ath-MIR2939, targets a pollen-specific F-box transcript and we demonstrate cleavage of its target mRNA in mature pollen. CONCLUSIONS: Despite the apparent simplicity of the male gametophyte, comprising just two different cell types, pollen not only utilises many miRNAs and trans-acting siRNAs expressed in the somatic tissues but also expresses novel miRNAs.

Peer Review status:Peer ReviewedPublication status:PublishedVersion:Publisher's version Funder: Biotechnology and Biological Sciences Research Council   Notes:Copyright 2009 Grant-Downton et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Bibliographic Details

Publisher: BioMed Central Ltd.

Publisher Website: http://www.biomedcentral.com/

Journal: BMC genomicssee more from them

Publication Website: http://www.biomedcentral.com/bmcgenomics

Issue Date: 2009

pages:Article no. 643Identifiers

Urn: uuid:51cf5b3b-6eab-4875-a33e-621e1aba793b

Source identifier: 53216

Eissn: 1471-2164

Doi: https://doi.org/10.1186/1471-2164-10-643

Issn: 1471-2164 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Arabidopsis Pollen MicroRNAs RNA, Small Interfering RNA, Plant Sequence Analysis, RNA Base Sequence Nucleic Acid Conformation Gene Expression Regulation, Plant Molecular Sequence Data Computational Biology Tiny URL: pubs:53216

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Author: Grant-Downton, R - institutionUniversity of Oxford Oxford, Mathematical,Physical and Life Sciences Division, Plant Sciences - - -

Source: https://ora.ox.ac.uk/objects/uuid:51cf5b3b-6eab-4875-a33e-621e1aba793b



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