Human cellular microRNA hsa-miR-29a interferes with viral nef protein expression and HIV-1 replicationReport as inadecuate




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Reference: Ahluwalia, JK, Khan, S, Soni, K et al., (2008). Human cellular microRNA hsa-miR-29a interferes with viral nef protein expression and HIV-1 replication. Retrovirology, 5 (1), Article: 117.Citable link to this page:

 

Human cellular microRNA hsa-miR-29a interferes with viral nef protein expression and HIV-1 replication

Abstract: Background: Cellular miRNAs play an important role in the regulation of gene expression in eukaryotes. Recently, miRNAs have also been shown to be able to target and inhibit viral gene expression. Computational predictions revealed earlier that the HIV-1 genome includes regions that may be potentially targeted by human miRNAs. Here we report the functionality of predicted miR-29a target site in the HIV-1 nef gene.Results: We find that the human miRNAs hsa-miR-29a and 29b are expressed in human peripheral blood mononuclear cells. Expression of a luciferase reporter bearing the nef miR-29a target site was decreased compared to the luciferase construct without the target site. Locked nucleic acid modified anti-miRNAs targeted against hsa-miR-29a and 29b specifically reversed the inhibitory effect mediated by cellular miRNAs on the target site. Ectopic expression of the miRNA results in repression of the target Nef protein and reduction of virus levels.Conclusion: Our results show that the cellular miRNA hsa-miR29a downregulates the expression of Nef protein and interferes with HIV-1 replication.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Council of Scientific and Industrial Research   Notes:© 2008 Ahluwalia et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Bibliographic Details

Publisher: BioMed Central Ltd.

Publisher Website: http://www.biomedcentral.com/

Journal: Retrovirologysee more from them

Publication Website: http://www.retrovirology.com/

Issue Date: 2008-12-23

pages:Article: 117Identifiers

Urn: uuid:5c51313d-8446-48e8-a90e-390255cf176b

Source identifier: 475155

Doi: https://doi.org/10.1186/1742-4690-5-117

Issn: 1742-4690 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: HIV (Viruses) Human biology Non-coding RNA Reproduction RNA-protein interactions Viral proteinsSubjects: Viruses Tiny URL: pubs:475155

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Author: Ahluwalia, JK - - - Khan, S - - - Soni, K - - - Rawat, P - - - Gupta, A - - - Hariharan, M - - - Scaria, V - - - Lalwani, M - ins

Source: https://ora.ox.ac.uk/objects/uuid:5c51313d-8446-48e8-a90e-390255cf176b



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