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Reference: Sadarangani, M, Hoe, JC, Makepeace, K et al., (2016). Phase variation of Opa proteins of Neisseria meningitidis and the effects of bacterial transformation. Journal of Biosciences, 1-7.Citable link to this page:

 

Phase variation of Opa proteins of Neisseria meningitidis and the effects of bacterial transformation

Abstract: Opa proteins are major proteins involved in meningococcal colonization of the nasopharynx and immune interactions. Opa proteins undergo phase variation (PV) due to the presence of the 5′-CTCTT-3′ coding repeat (CR) sequence. The dynamics of PV of meningococcal Opa proteins is unknown. Opa PV, including the effect of transformation on PV, was assessed using a panel of Opa-deficient strains of Neisseria meningitidis. Analysis of Opa expression from UK disease-causing isolates was undertaken. Different opa genes demonstrated variable rates of PV, between 6.4 ×10–4 and 6.9 ×10–3 per cell per generation. opa genes with a longer CR tract had a higher rate of PV (r2=0.77, p=0.1212). Bacterial transformation resulted in a 180-fold increase in PV rate. The majority of opa genes in UK disease isolates (315/463, 68.0%) were in the ‘on’ phase, suggesting the importance of Opa proteins during invasive disease. These data provide valuable information for the first time regarding meningococcal Opa PV. The presence of Opa PV in meningococcal populations and high expression of Opa among invasive strains likely indicates the importance of this protein in bacterial colonization in the human nasopharynx. These findings have potential implications for development of vaccines derived from meningococcal outer membranes.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Action Medical Research   Notes:© Indian Academy of Sciences

Bibliographic Details

Publisher: Indian Academy of Sciences

Publisher Website: http://www.ias.ac.in/

Journal: Journal of Biosciencessee more from them

Publication Website: http://link.springer.com/journal/12038

Issue Date: 2016-01

pages:1-7Identifiers

Urn: uuid:60580d50-74a1-4b4a-b628-4a6512d991a0

Source identifier: 598431

Eissn: 0973-7138

Doi: https://doi.org/10.1007/s12038-016-9588-y

Issn: 0250-5991 Item Description

Type: Journal article;

Version: Publisher's versionKeywords: Colonization Meningococcus Opa protein Phase variation Transformation Tiny URL: pubs:598431

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Author: Sadarangani, M - institutionUniversity of Oxford Oxford, MSD, Paediatrics - - - Hoe, JC - - - Makepeace, K - - - van der Ley, P -

Source: https://ora.ox.ac.uk/objects/uuid:60580d50-74a1-4b4a-b628-4a6512d991a0



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