Three-dimensional histology: tools and application to quantitative assessment of cell-type distribution in rabbit heart.Report as inadecuate




Three-dimensional histology: tools and application to quantitative assessment of cell-type distribution in rabbit heart. - Download this document for free, or read online. Document in PDF available to download.

Reference: Burton, RA, Lee, P, Casero, R et al., (2014). Three-dimensional histology: tools and application to quantitative assessment of cell-type distribution in rabbit heart. Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology, 16 Suppl 4 (suppl 4), iv86-iv95.Citable link to this page:

 

Three-dimensional histology: tools and application to quantitative assessment of cell-type distribution in rabbit heart.

Abstract: AIMS: Cardiac histo-anatomical organization is a major determinant of function. Changes in tissue structure are a relevant factor in normal and disease development, and form targets of therapeutic interventions. The purpose of this study was to test tools aimed to allow quantitative assessment of cell-type distribution from large histology and magnetic resonance imaging- (MRI) based datasets. METHODS AND RESULTS: Rabbit heart fixation during cardioplegic arrest and MRI were followed by serial sectioning of the whole heart and light-microscopic imaging of trichrome-stained tissue. Segmentation techniques developed specifically for this project were applied to segment myocardial tissue in the MRI and histology datasets. In addition, histology slices were segmented into myocytes, connective tissue, and undefined. A bounding surface, containing the whole heart, was established for both MRI and histology. Volumes contained in the bounding surface (called 'anatomical volume'), as well as that identified as containing any of the above tissue categories (called 'morphological volume'), were calculated. The anatomical volume was 7.8 cm(3) in MRI, and this reduced to 4.9 cm(3) after histological processing, representing an 'anatomical' shrinkage by 37.2%. The morphological volume decreased by 48% between MRI and histology, highlighting the presence of additional tissue-level shrinkage (e.g. an increase in interstitial cleft space). The ratio of pixels classified as containing myocytes to pixels identified as non-myocytes was roughly 6:1 (61.6 vs. 9.8%; the remaining fraction of 28.6% was 'undefined'). CONCLUSION: Qualitative and quantitative differentiation between myocytes and connective tissue, using state-of-the-art high-resolution serial histology techniques, allows identification of cell-type distribution in whole-heart datasets. Comparison with MRI illustrates a pronounced reduction in anatomical and morphological volumes during histology processing.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Biotechnology and Biological Sciences Research Council   Funder: European Research Council   Notes:Copyright information © The Author 2014. Published by Oxford University Press on behalf of the European Society of Cardiology. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions[at]oup.com

Bibliographic Details

Publisher: Oxford University Press

Publisher Website: http://www.oxfordjournals.org/

Journal: Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiologysee more from them

Publication Website: http://europace.oxfordjournals.org/

Issue Date: 2014-11

pages:iv86-iv95Identifiers

Urn: uuid:ae349137-0294-4ea3-8a80-17f17d9265ae

Source identifier: 488422

Eissn: 1532-2092

Doi: https://doi.org/10.1093/europace/euu234

Issn: 1099-5129 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Myocardium Heart Myocytes, Cardiac Animals Rabbits Image Interpretation, Computer-Assisted Imaging, Three-Dimensional Magnetic Resonance Imaging Heart Arrest, Induced Models, Animal Models, Cardiovascular Computer Graphics Computer Simulation Female Tiny URL: pubs:488422

Relationships





Author: Burton, RA - institutionUniversity of Oxford Oxford, MSD, Physiology Anatomy and Genetics - - - Lee, P - - - Casero, R - institut

Source: https://ora.ox.ac.uk/objects/uuid:ae349137-0294-4ea3-8a80-17f17d9265ae



DOWNLOAD PDF




Related documents