Short-term starvation of immune deficient Drosophila improves survival to gram-negative bacterial infections.Report as inadecuate




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Reference: Brown, AE, Baumbach, J, Cook, PE et al., (2009). Short-term starvation of immune deficient Drosophila improves survival to gram-negative bacterial infections. PloS one, 4 (2), Article: e4490.Citable link to this page:

 

Short-term starvation of immune deficient Drosophila improves survival to gram-negative bacterial infections.

Abstract: BACKGROUND: Primary immunodeficiencies are inborn errors of immunity that lead to life threatening conditions. These predispositions describe human immunity in natura and highlight the important function of components of the Toll-IL-1- receptor-nuclear factor kappa B (TIR-NF-kappaB) pathway. Since the TIR-NF-kappaB circuit is a conserved component of the host defence in higher animals, genetically tractable models may contribute ideas for clinical interventions. METHODOLOGY/PRINCIPAL FINDINGS: We used immunodeficient fruit flies (Drosophila melanogaster) to address questions pertaining to survival following bacterial infection. We describe here that flies lacking the NF-kappaB protein Relish, indispensable for countering Gram-negative bacteria, had a greatly improved survival to such infections when subject to dietary short-term starvation (STS) prior to immune challenge. STS induced the release of Nitric Oxide (NO), a potent molecule against pathogens in flies, mice and humans. Administering the NO Synthase-inhibitory arginine analog N-Nitro-L-Arginine-Methyl-Ester (L-NAME) but not its inactive enantiomer D-NAME increased once again sensitivity to infection to levels expected for relish mutants. Surprisingly, NO signalling required the NF-kappaB protein Dif, usually needed for responses against Gram-positive bacteria. CONCLUSIONS/SIGNIFICANCE: Our results show that NO release through STS may reflect an evolutionary conserved process. Moreover, STS could be explored to address immune phenotypes related to infection and may offer ways to boost natural immunity.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: EPA Cephalosporin Trust Fund   Funder: Medical Research Council   Notes:Copyright 2009 Brown et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Public Library of Science

Publisher Website: http://www.plos.org

Journal: PloS onesee more from them

Publication Website: http://www.plosone.org

Issue Date: 2009

pages:Article: e4490Identifiers

Urn: uuid:d2a6af61-369b-4cca-bc97-4ab9c71759a0

Source identifier: 99930

Eissn: 1932-6203

Doi: https://doi.org/10.1371/journal.pone.0004490

Issn: 1932-6203 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Animals Humans Mice Gram-Negative Bacterial Infections Starvation NG-Nitroarginine Methyl Ester Transcription Factors NF-kappa B Drosophila Proteins Enzyme Inhibitors Survival Rate Signal Transduction Anti-Infective Agents Nitric Oxide Drosophila melanogaster Antimicrobial Cationic Peptides Nitric Oxide Synthase Tiny URL: pubs:99930

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Author: Brown, AE - institutionUniversity of Oxford - - - Baumbach, J - institutionUniversity of Oxford fundingStudienstiftung des Deutsc

Source: https://ora.ox.ac.uk/objects/uuid:d2a6af61-369b-4cca-bc97-4ab9c71759a0



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