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Reference: Diepold, A, Kudryashev, M, Delalez, NJ et al., (2015). Composition, formation, and regulation of the cytosolic c-ring, a dynamic component of the type III secretion injectisome. PLoS Biology, 13 (1), e1002039.Citable link to this page:

 

Composition, formation, and regulation of the cytosolic c-ring, a dynamic component of the type III secretion injectisome

Abstract: Many gram-negative pathogens employ a type III secretion injectisome to translocate effector proteins into eukaryotic host cells. While the structure of the distal needle complex is well documented, the composition and role of the functionally important cytosolic complex remain less well understood. Using functional fluorescent fusions, we found that the C-ring, an essential and conserved cytosolic component of the system, is composed of ~22 copies of SctQ (YscQ in Yersinia enterocolitica), which require the presence of YscQC, the product of an internal translation initiation site in yscQ, for their cooperative assembly. Photoactivated localization microscopy (PALM) reveals that in vivo, YscQ is present in both a free-moving cytosolic and a stable injectisome-bound state. Notably, fluorescence recovery after photobleaching (FRAP) shows that YscQ exchanges between the injectisome and the cytosol, with a t½ of 68 ± 8 seconds when injectisomes are secreting. In contrast, the secretin SctC (YscC) and the major export apparatus component SctV (YscV) display minimal exchange. Under non-secreting conditions, the exchange rate of YscQ is reduced to t½ = 134 ± 16 seconds, revealing a correlation between C-ring exchange and injectisome activity, which indicates a possible role for C-ring stability in regulation of type III secretion. The stabilization of the C-ring depends on the presence of the functional ATPase SctN (YscN). These data provide new insights into the formation and composition of the injectisome and present a novel aspect of type III secretion, the exchange of C-ring subunits, which is regulated with respect to secretion.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's versionNotes:Copyright © 2015 Diepold et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Public Library of Science

Publisher Website: http://www.plos.org/

Journal: PLoS Biologysee more from them

Publication Website: http://www.plosbiology.org/

Issue Date: 2015-1

pages:e1002039Identifiers

Urn: uuid:dda7bae2-187a-4f00-9e16-9f35165c78cb

Source identifier: 504583

Eissn: 1545-7885

Doi: https://doi.org/10.1371/journal.pbio.1002039

Issn: 1544-9173 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: secretion flagella fluorescence imaging fluorescence recovery after photobleaching fluorescence microscopy cell fusion yersinia enterocolitica yersinia Tiny URL: pubs:504583

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Author: Diepold, A - institutionUniversity of Oxford Oxford, MSD, Biochemistry - - - Kudryashev, M - - - Delalez, NJ - institutionUnivers

Source: https://ora.ox.ac.uk/objects/uuid:dda7bae2-187a-4f00-9e16-9f35165c78cb



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