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Reference: Morteau, O, Blundell, S, Chakera, A et al., (2010). Renal transplant immunosuppression impairs natural killer cell function in vitro and in vivo. PloS one, 5 (10), Article: e13294.Citable link to this page:

 

Renal transplant immunosuppression impairs natural killer cell function in vitro and in vivo.

Abstract: BACKGROUND: Despite an increasing awareness of the importance of innate immunity, the roles of natural killer (NK) cells in transplant rejection and antiviral and cancer immunity during immunosuppression have not been clearly defined. METHODS: To address this issue we have developed a quantitative assay of NK cell function that can be used on clinical samples and have studied the influence of immunosuppression on NK cell function. NK cell degranulation and intracellular interferon (IFN)-γ production were determined by flow cytometry of peripheral blood samples. RESULTS: Overnight ex vivo treatment of peripheral blood cells from healthy controls with ciclosporin or tacrolimus inhibited NK cell degranulation and IFN-γ production in a dose-dependent manner. A similar impairment of function was seen in NK cells from patients treated in vivo with calcineurin inhibitors. In the early post-transplant period, there was a variable reduction of NK cell counts after treatment with alemtuzumab and basiliximab. CONCLUSIONS: The functional inhibition of NK cells in early transplant patients coincides with the period of maximum susceptibility to viral infections. The ability to assay NK cell function in clinical samples allows assessment of the impact of immunosuppression on these effector cells. This information may be helpful in guiding the titration of immunosuppression in the clinical setting.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: National Institute for Health Research, Oxford Comprehensive Biomedical Research Centre Programme   Notes:Copyright 2010 Morteau et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Public Library of Science

Publisher Website: http://www.plos.org

Journal: PloS onesee more from them

Publication Website: http://www.plosone.org

Issue Date: 2010

pages:Article: e13294Identifiers

Urn: uuid:e927ce61-93dc-47fa-a2b3-261dfd89b673

Source identifier: 90805

Eissn: 1932-6203

Doi: https://doi.org/10.1371/journal.pone.0013294

Issn: 1932-6203 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Humans Cyclosporine Immunosuppressive Agents Flow Cytometry Cell Degranulation Killer Cells, Natural Kidney Transplantation Tacrolimus Interferon-gamma Tiny URL: pubs:90805

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Author: Morteau, O - institutionUniversity of Oxford - - - Blundell, S - institutionUniversity of Oxford - - - Chakera, A - institutionUn

Source: https://ora.ox.ac.uk/objects/uuid:e927ce61-93dc-47fa-a2b3-261dfd89b673



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