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Reference: Walsh, E, Feuerborn, A and Cook, PR, (2016). Formation of droplet interface bilayers in a Teflon tube. Scientific Reports.Citable link to this page:

 

Formation of droplet interface bilayers in a Teflon tube.

Abstract: Droplet-interface bilayers (DIBs) have applications in disciplines ranging from biology to computing. We present a method for forming them manually using a Teflon tube attached to a syringe pump; this method is simple enough it should be accessible to those without expertise in microfluidics. It exploits the properties of interfaces between three immiscible liquids, and uses fluid flow through the tube to pack together drops coated with lipid monolayers to create bilayers at points of contact. It is used to create functional nanopores in DIBs composed of phosphocholine using the protein α-hemolysin (αHL), to demonstrate osmotically-driven mass transfer of fluid across surfactant-based DIBs, and to create arrays of DIBs. The approach is scalable, and thousands of DIBs can be prepared using a robot in one hour; therefore, it is feasible to use it for high throughput applications.

Publication status:PublishedPeer Review status:Peer reviewedVersion:Publisher's versionDate of acceptance:2016-09-09 Funder: Medical Research Council   Funder: European Research Council   Funder: John Fell Fund   Notes:© the Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License.

Bibliographic Details

Publisher: Nature Publishing Group

Publisher Website: http://www.nature.com/

Journal: Scientific Reportssee more from them

Publication Website: http://www.nature.com/srep/index.html

Volume: 6

Extent: 34355

Issue Date: 2016-09-29Identifiers

Doi: https://doi.org/10.1038/srep34355

Uuid: uuid:0816077d-a63c-473f-9d6c-1fe6afd55677

Urn: uri:0816077d-a63c-473f-9d6c-1fe6afd55677

Pubs-id: pubs:647918

Issn: 2045-2322

Eissn: 2045-2322 Item Description

Type: journal-article;

Language: eng

Version: Publisher's versionKeywords: Biomedical engineering Chemical engineering

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Author: Walsh, E - Oxford, MPLS, Engineering Science - - - Feuerborn, A - - - Cook, PR - - - - Bibliographic Details Publisher: Nature Pu

Source: https://ora.ox.ac.uk/objects/uuid:0816077d-a63c-473f-9d6c-1fe6afd55677



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