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Reference: Ersek, A, Espirito Santo, AI, Vattakuzhi, Y et al., (2016). Strain dependent differences in glucocorticoid-induced bone loss between C57BL/6J and CD-1 mice. Scientific Reports, 6, Article: 36513.Citable link to this page:

 

Strain dependent differences in glucocorticoid-induced bone loss between C57BL/6J and CD-1 mice

Abstract: We have investigated the effect of long-term glucocorticoid (GC) administration on bone turnover in two frequently used mouse strains; C57BL/6J and CD1, in order to assess the influence of their genetic background on GC-induced osteoporosis (GIO). GIO was induced in 12 weeks old female C57BL/6J and CD1 mice by subcutaneous insertion of long-term release prednisolone or placebo pellets. Biomechanical properties as assessed by three point bent testing revealed that femoral elasticity and strength significantly decreased in CD1 mice receiving GC, whereas C57BL/6J mice showed no differences between placebo and prednisolone treatment. Bone turnover assessed by microcomputer tomography revealed that contrary to C57BL/6J mice, prednisolone treated CD1 mice developed osteoporosis. In vitro experiments have underlined that, at a cellular level, C57BL/6J mice osteoclasts and osteoblasts were less responsive to GC treatment and tolerated higher doses than CD1 cells. Whilst administration of long-term release prednisolone pellets provided a robust GIO animal model in 12 weeks old CD1 mice, age matched C57BL/6J mice were not susceptible to the bone changes associated with GIO. This study indicates that for the induction of experimental GIO, the mouse strain choice together with other factors such as age should be carefully evaluated.

Publication status:PublishedPeer Review status:Peer reviewedVersion:Publisher's VersionDate of acceptance:30 September 2016 Funder: Arthritis Research UK   Funder: Kennedy Institute of Rheumatology   Notes:Copyright © 2016 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The imagesor other third party material in this article are included in the article’s Creative Commons license,unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license,users will need to obtain permission from the license holder to reproduce the material. To view a copy of thislicense, visit http://creativecommons.org/licenses/by/4.0/

Bibliographic Details

Publisher: Nature Publishing Group

Publisher Website: http://www.nature.com/

Journal: Scientific Reportssee more from them

Publication Website: http://www.nature.com/srep/

Volume: 6

Extent: Article: 36513

Issue Date: 2016-11-04

pages:Article: 36513Identifiers

Doi: https://doi.org/10.1038/srep36513

Uuid: uuid:0a75109d-7c1d-49d6-ba4e-036f0591adf5

Urn: uri:0a75109d-7c1d-49d6-ba4e-036f0591adf5

Pubs-id: pubs:656489

Issn: 2045-2322 Item Description

Type: journal-article;

Version: Publisher's VersionKeywords: glucocorticoids osteoporosis murine bones

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Author: Ersek, A - Oxford, MSD, NDORMS, KIR - - - Espirito Santo, AI - Oxford, MSD, NDORMS, KIR - - - Vattakuzhi, Y - - - George, S - Oxf

Source: https://ora.ox.ac.uk/objects/uuid:0a75109d-7c1d-49d6-ba4e-036f0591adf5



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