Screening for endophytic nitrogen-fixing bacteria in Brazilian sugar cane varieties used in organic farming and description of Stenotrophomonas pavanii sp. nov.Report as inadecuate




Screening for endophytic nitrogen-fixing bacteria in Brazilian sugar cane varieties used in organic farming and description of Stenotrophomonas pavanii sp. nov. - Download this document for free, or read online. Document in PDF available to download.

(2011)INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY.61(4).p.926-931 Mark abstract In a study investigating the biodiversity of endophytic nitrogen-fixing bacteria in Brazilian sugarcane plants, 31 isolates were obtained from roots, stems and leaves. Nitrogen fixation capability was determined by the acetylene reduction assay (ARA) and the presence of the nifH gene sequence was detected by dot-blot hybridization. RFLP-PCR patterns and 16S rRNA gene sequence analysis revealed the presence of 11 different bacterial genera. One strain (ICB 89T), belonging to the genus Stenotrophomonas, was further investigated using a polyphasic taxonomic approach. Strain ICB 89T is a Gram-negative, rod-shaped, non-spore-forming and nitrogen-fixing bacterium and was isolated from stems of a Brazilian sugarcane variety widely used in organic farming. 16S rRNA gene sequence analysis and DNA-DNA hybridizations revealed that strain ICB 89T is genotypically different from the closest related Stenotrophomonas species, namely S. maltophilia, S. rhizophila and S. nitritireducens. Chemotaxonomic data and biochemical characteristics allow the differentiation of strain ICB 89T from its phylogenetically nearest neighbours. Strain ICB 89T therefore represents a new species, for which the name Stenotrophomonas pavanii sp. nov. is proposed, with the type strain ICB 89T (CBMAI 564T = LMG 25348T).

Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1105297



Author: Patricia L Ramos, Stefanie Van Trappen, Fabiano L Thompson, Rafael CS Rocha, Heloiza R Barbosa, Paul De Vos and Carlos A Moreira-F

Source: https://biblio.ugent.be/publication/1105297



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