Role of anti-domain 1-beta2glycoprotein I antibodies in the diagnosis and risk stratification of antiphospholipid syndromeReport as inadecuate




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(2016)JOURNAL OF THROMBOSIS AND HAEMOSTASIS.14(9).p.1779-1787 Mark abstract Background: Laboratory diagnosis of antiphospholipid syndrome (APS) includes lupus anticoagulant (LAC), anticardiolipin (aCL) or anti-beta(2) glycoprotein I (a beta(2)GPI) antibodies. Antibodies targeting domain 1 of beta(2)GPI (aD1) constitute a pathogenic subset of autoantibodies. Objectives: In this cohort study, we determined the clinical performance characteristics, additional diagnostic value and the contribution to APS risk stratification of an automated aD1 assay. Patients/Methods: LAC, aCL, a beta(2)GPI and aD1 IgG were measured in 101 APS patients, 123 patients with autoimmune disorders, 82 diseased controls and 120 healthy controls. aD1 antibodies were detected by QUANTA Flash (R) Beta2GPI-Domain 1 chemiluminescence immunoassay. Results: With a cut-off value of 20.0 CU, the aD1 IgG assay identifies APS patients in a clinically affected patient cohort with a sensitivity of 53.5% and specificity of 98.8%. It implied a high odds ratio (OR) for clinical events (OR, 17.0; 95% confidence interval [CI], 7.1-40.5). aD1 IgG did not add diagnostic value to the formal aPL panel because a beta(2)GPI IgG was nearly as specific but more sensitive for APS (sensitivity 56.4%) with a higher OR for clinical events (36.2; 95% CI, 11.1-117.9). High aD1 titers identify triple- positive patients and patients with thrombosis in a beta(2)GPI-dependent LAC-positive population. Agreement between aD1 IgG and a beta(2)GPI IgG was high (positive and negative agreement 91.7% and 98.4%, respectively). Conclusion: Detection of aD1 IgG correctly classifies patients at risk of thrombosis. However, the contribution of aD1 IgG to APS diagnosis and risk stratification depends upon the solid phase assays used for aCL and a beta(2)GPI detection.

Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8528606



Author: Ann-Sophie De Craemer, J. Musial and Katrien Devreese

Source: https://biblio.ugent.be/publication/8528606



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