A COMPARATIVE STUDY OF METHODS OF SOMATIC CELL FUSION.Report as inadecuate


 A COMPARATIVE STUDY OF METHODS OF SOMATIC CELL FUSION.


A COMPARATIVE STUDY OF METHODS OF SOMATIC CELL FUSION. - Download this document for free, or read online. Document in PDF available to download.



Type of Resource: text

Genre: Electronic Thesis or Dissertation

Issuance: monographic

Date Issued: 1975

Physical Form: pdf

Extent: 85 p.

Language(s): English

Summary: The effectiveness of polyethylene glycol as a fusogenicagent tor avian erythrocytes was evaluated. Parametersaffecting the efficiency of fusion included number of pre-fusionwashes, age of cells, length of exposure to enzymesolutions at varied pH values, composition of wash solutionsand molecular weights of polyethylene glycol. Fusion wasmeasured as the percentage of visible polykaryons. Maximalfusion obtained using polyethylene glycol was approximately60-70%. Other methods of cell fusion (lysolecithin, Sendaivirus, high calcium concentration at high pH) were comparedto polyethylene glycol. All other means yielded lesseramounts of fused products. A photographic comparison of thevarious methods is presented, and possible mechanisms offusion are discussed.

Identifier: 13703 (digitool), FADT13703 (IID), fau:10535 (fedora)

Note(s): Thesis (M.S.)--Florida Atlantic University, 1975.

Subject(s): Biology, General

Persistent Link to This Record: http://purl.flvc.org/fcla/dt/13703

Owner Institution: FAU



Author: GALLA, JAN DAVID. Florida Atlantic University

Source: http://fau.digital.flvc.org/islandora/object/fau%3A10535



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