V-J combinations of T-cell receptor predict responses to erythropoietin in end-stage renal disease patientsReport as inadecuate




V-J combinations of T-cell receptor predict responses to erythropoietin in end-stage renal disease patients - Download this document for free, or read online. Document in PDF available to download.

Journal of Biomedical Science

, 24:43

First Online: 11 July 2017Received: 01 January 2017Accepted: 26 June 2017

Abstract

BackgroundAnemia is common among end-stage renal disease ESRD patients who undergone hemodialysis. The total reduction of red blood cell RBC count is associated with poor prognosis in these patients. Although erythropoietin EPO has been used as an effective treatment for ESRD patients with anemia, a large number of patients still present poor responses to EPO treatment.

MethodsWe measured T-cell receptor sequencing profiles, including length of complementarity-deteremining region 3 CDR3, intra- and inter-group EPO resistant vs. responsive clonotype diversity, VDJ usage profiles and V-J combinations from ESRD patients and to investigate the correlation between these features and EPO treatment efficacy.

ResultsOur results revealed statistical significance in the top 3 ~ 15 most abundant joint distributions of Vβ-Jβ among the two groups, suggesting the importance of V or J gene utilization in the EPO response of ESRD patients.

ConclusionsIn summary, we provided evidence addressing the potential correlation between the immune repertoire and EPO response in ESRD patients.

Trial registrationTMU-JIRB 201309026. Registered 16 October 2013.

KeywordsEnd-stage renal disease Erythropoietin treatment T-cell receptor repertoire High-throughput sequencing AbbreviationsBpBase-pair

CDR3Complementarity-determining region 3

CKDChronic kidney disease

EPOErythropoietin

ERIErythropoietin resistance index

ESAsErythropoiesis-stimulating agents

ESRDEnd-stage renal disease

GFRGlomerular filtration rate

HbHemoglobin

HLAHuman leukocyte antigen

HTSHigh-throughput sequencing

IFNInterferon

ILInterleukin

PBMCsPeripheral blood mononuclear cells

PBSPhosphate-buffered saline

PCAPrincipal component analysis

PCRPolymerase chain reaction

RBCRed blood cell

RDIRepertoire diversity index

RTReverse-transcription

SDStandard deviation

TCRT-cell receptor

TNFTumor necrosis factor

USRDSUnited States Renal Data System

Electronic supplementary materialThe online version of this article doi:10.1186-s12929-017-0349-5 contains supplementary material, which is available to authorized users.

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Author: Henry Sung-Ching Wong - Che-Mai Chang - Chih-Chin Kao - Yu-Wen Hsu - Xiao Liu - Wen-Chang Chang - Mai-Szu Wu - Wei-Chiao 

Source: https://link.springer.com/



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