CD4 CD25 CD127− and CD4 CD25 Foxp3 Regulatory T Cell Subsets in Mediating Autoimmune Reactivity in Systemic Lupus Erythematosus PatientsReport as inadecuate




CD4 CD25 CD127− and CD4 CD25 Foxp3 Regulatory T Cell Subsets in Mediating Autoimmune Reactivity in Systemic Lupus Erythematosus Patients - Download this document for free, or read online. Document in PDF available to download.

Archivum Immunologiae et Therapiae Experimentalis

, Volume 64, Issue 5, pp 399–407

First Online: 07 May 2016Received: 04 August 2015Accepted: 10 January 2016

Abstract

The available clinical as well as experimental studies implicate participation of T regulatory Treg subsets in the pathogenesis and course of systemic lupus erythematosus SLE. Introduction of the CD4CD25CD127 and CD4CD25Foxp3 regulatory subpopulations analysis into immunological processes assessment and disease activation prognosis in patients with lupus nephritis LN may improve monitoring of disease activity and enable an early, and thus more effective, therapeutic treatment. The main goal of the study was to investigate whether the quantitative changes of Treg subpopulations are related to the clinical status of patients with LN. Fifty-four adult SLE patients divided into two groups according to their SLEDAI and renal SLEDAI scores were enrolled into the study. Subpopulations of CD4CD25CD127 and CD4CD25Foxp3 phenotypes were determined by flow cytometry. The control group had higher absolute number of CD4CD25Foxp3 cells compared with the study group p < 0.001. Also, significant inverse correlation in the absolute number of CD4CD25Foxp3 cells and SLEDAI score was observed. There were significant differences in the percentage and absolute number of CD4CD25Foxp3 lymphocytes between active and non-active LN groups. The study group had statistically lower values of CD4CD25CD127 cells, both in the percentage p < 0.001 as well as their absolute number p = 0.014 compared to the control group. There were also statistically significant positive correlations between the absolute number of CD4CD25CD127 and CD4CD25Foxp3 Tregs. In conclusion: 1 reduction in the number of regulatory CD4CD25Foxp3 cells is a promising indicator of the activity of SLE, particularly of renal involvement; 2 determination of the number of regulatory cells using the CD4CD25CD127 phenotype is unreliable in patients with SLE.

KeywordsSystemic lupus erythematosus Lupus nephritis Regulatory cells Flow cytometry AbbreviationsAnti-dsDNAAnti-double stranded DNA antibodies

CDCluster of differentiation

Foxp3Transcription factor forkhead box P3

HIVHuman immunodeficiency virus

LNLupus nephritis

mRNAMessenger RNA

rSLEDAIRenal systemic lupus erythematosus disease activity index

SLESystemic lupus erythematosus

SLEDAISystemic lupus erythematosus disease activity index

TeffEffector T lymphocyte

TregRegulatory T lymphocyte

PBSPhosphate-buffered saline

FBSFetal bovine serum

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Author: Marcelina Żabińska - Magdalena Krajewska - Katarzyna Kościelska-Kasprzak - Katarzyna Jakuszko - Dorota Bartoszek - Marta 

Source: https://link.springer.com/







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