Dietary green-plant thylakoids decrease gastric emptying and gut transit, promote changes in the gut microbial flora, but does not cause steatorrheaReport as inadecuate




Dietary green-plant thylakoids decrease gastric emptying and gut transit, promote changes in the gut microbial flora, but does not cause steatorrhea - Download this document for free, or read online. Document in PDF available to download.

Nutrition and Metabolism

, 13:67

First Online: 12 October 2016Received: 09 May 2016Accepted: 03 October 2016

Abstract

Green-plant thylakoids increase satiety by affecting appetite hormones such as ghrelin, cholecystokinin CCK and glucagon-like peptide-1 GLP-1. The objective of this study was to investigate if thylakoids also affect gastrointestinal GI passage and microbial composition. To analyse the effects on GI passage, 16 rats were gavage-fed a control or thylakoid-supplemented high-fat diet HFD 30 min before receiving Evans blue. Another 16 rats were fed a control HFD or thylakoid HFD for two weeks prior to the intragastric challenge with Evans blue. The amount of Evans blue in the stomach and the distance of migration in the intestines after 30 min were used as a measurement of gastric emptying and intestinal transit. These were reduced by thylakoid supplementation in the acute study, and however not significantly also after the two-week diet study.

The second aim of the study was to investigate if thylakoid-supplementation affects the gut microbiota and amount of faecal fat in healthy human volunteers n = 34 receiving thylakoid or placebo treatments for three months. Microbiota was analysed using 16S rRNA gene sequencing and qPCR, and faecal fat was extracted by dichloromethane. The total bacteria, and specifically the Bacteriodes fragilis group, were increased by thylakoid treatment versus placebo, while thylakoids did not cause steatorrhea. Dietary supplementation with thylakoids thus affects satiety both via appetite hormones and GI fullness, and affects the microbial composition without causing GI adverse effects such as steatorrhea. This suggests thylakoids as a novel agent in prevention and treatment of obesity.

KeywordsIntestinal transit Microbiota Faecal fat Spinach Obesity AbbreviationsCCKCholecystokinin

DCMDichloromethane

GEGastric emptying

GIGastrointestinal

GLP-1Glucagon-like peptide-1

HFDHigh-fat diet

MiGastrointestinal migration

PYYPeptide tyrosin-tyrosin

RQRespiratory quotient

rRNARibosomal RNA

SCFAShort-chain fatty acids

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Author: Eva-Lena Stenblom - Björn Weström - Caroline Linninge - Peter Bonn - Mary Farrell - Jens F. Rehfeld - Caroline Montelius

Source: https://link.springer.com/



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