Helicobacter suis affects the health and function of porcine gastric parietal cellsReport as inadecuate




Helicobacter suis affects the health and function of porcine gastric parietal cells - Download this document for free, or read online. Document in PDF available to download.

Veterinary Research

, 47:101

First Online: 19 October 2016Received: 03 August 2016Accepted: 14 September 2016

Abstract

The stomach of pigs at slaughter age is often colonized by Helicobacter H. suis, which is also the most prevalent gastric non-H. pylori Helicobacter NHPH species in humans. It is associated with chronic gastritis, gastric ulceration and other gastric pathological changes in both hosts. Parietal cells are highly specialized, terminally differentiated epithelial cells responsible for gastric acid secretion and regulation. Dysfunction of these cells is closely associated with gastric pathology and disease. Here we describe a method for isolation and culture of viable and responsive parietal cells from slaughterhouse pigs. In addition, we investigated the interactions between H. suis and gastric parietal cells both in H. suis-infected six-month-old slaughter pigs, as well as in our in vitro parietal cell model. A close interaction of H. suis and parietal cells was observed in the fundic region of stomachs from H. suis positive pigs. The bacterium was shown to be able to directly interfere with cultured porcine parietal cells, causing a significant impairment of cell viability. Transcriptional levels of Atp4a, essential for gastric acid secretion, showed a trend towards an up-regulation in H. suis positive pigs compared to H. suis-negative pigs. In addition, sonic hedgehog, an important factor involved in gastric epithelial differentiation, gastric mucosal repair, and stomach homeostasis, was also significantly up-regulated in H. suis positive pigs. In conclusion, this study describes a successful approach for the isolation and culture of porcine gastric parietal cells. The results indicate that H. suis affects the viability and function of this cell type.

Bram Flahou and Freddy Haesebrouck shared senior authorship

Download fulltext PDF



Author: Guangzhi Zhang - Richard Ducatelle - Belgacem Mihi - Annemieke Smet - Bram Flahou - Freddy Haesebrouck

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents