Differential expression and characterization of cypermethrin-degrading potential proteins in Bacillus thuringiensis strain, SG4Report as inadecuate




Differential expression and characterization of cypermethrin-degrading potential proteins in Bacillus thuringiensis strain, SG4 - Download this document for free, or read online. Document in PDF available to download.

3 Biotech

, 6:225

First Online: 19 October 2016Received: 30 July 2016Accepted: 03 October 2016

Abstract

A cypermethrin-degrading bacterium SG4 was isolated from the pesticide-contaminated soil in the agricultural field of the crop research centre of the University, and characterized as Bacillus thuringiensis strain, SG4. The bacterium degraded 78.9 % of cypermethrin 50 ppm in 15 days when grown in a minimal medium. To understand the functional proteins of cypermethrin degradation in Bacillus thuringiensis strain SG4, a comparative proteomic analysis was performed in the presence-absence of cypermethrin after 5 days of incubation in minimal medium. More than 450 spots corresponding to different proteins were recorded by 2D electrophoresis. We report expression of 223 and 250 unique proteins under normal and induced conditions cypermethrin stress, respectively. Identified proteins were categorized into different functional groups on the basis of their biological functions, viz., catabolic enzymes, translational and stress proteins, etc. Characterization of cypermethrin-specific proteins in a bacterial strain will help in biodegradation practices in situ.

KeywordsProteomics Cypermethrin Bacillus thuringiensis Two-dimensional gel electrophoresis Biodegradation  Download fulltext PDF



Author: Pankaj - Geeta Negi - Saurabh Gangola - Priyanka Khati - Govind Kumar - Anjana Srivastava - Anita Sharma

Source: https://link.springer.com/







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