Chikungunya virus transmission between Aedes albopictus and laboratory miceReport as inadecuate




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Parasites and Vectors

, 9:555

First Online: 19 October 2016Received: 12 June 2016Accepted: 06 October 2016

Abstract

BackgroundChikungunya virus CHIKV is a mosquito-borne alphavirus associated with epidemics of acute and chronic arthritic disease in humans. Aedes albopictus has emerged as an important new natural vector for CHIKV transmission; however, mouse models for studying transmission have not been developed.

MethodsAedes albopictus mosquitoes were infected with CHIKV via membrane feeding and by using infected adult wild-type C57BL-6 mice. Paraffin sections of infected mosquitoes were analysed by immunofluorescent antibody staining using an anti-CHIKV antibody. CHIKV-infected mosquitoes were used to infect adult C57BL-6 and interferon response factor 3 and 7 deficient IRF3-7 mice.

ResultsFeeding mosquitoes on blood meals with CHIKV titres > 5 log10CCID50-ml, either by membrane feeding or feeding on infected mice, resulted in  ≥ 50 % of mosquitoes becoming infected. However, CHIKV titres in blood meals  ≥ 7 log10CCID50-ml were required before salivary glands showed significant levels of immunofluorescent staining with an anti-CHIKV antibody. Mosquitoes fed on blood meals of 7.5 but not 5.9 log10CCID50-ml were able efficiently to transmit virus to adult C57BL-6 and IRF3-7 mice, with the latter mice showing overt signs of arthritis post-infection.

ConclusionsThe results provide a simple in vivo model for studying transmission of CHIKV from mosquitoes to mammals and also argue against a resistance barrier to CHIKV infection in adult mice.

KeywordsChikungunya virus Aedes albopictus Mouse model AbbreviationsCCIDCell culture infective dose

CHIKVChikungunya virus

IRFInterferon response factor

Electronic supplementary materialThe online version of this article doi:10.1186-s13071-016-1838-1 contains supplementary material, which is available to authorized users.

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Author: Leon E. Hugo - Natalie A. Prow - Bing Tang - Greg Devine - Andreas Suhrbier

Source: https://link.springer.com/







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