Yi Ai Fang, a traditional Chinese herbal formula, impacts the vasculogenic mimicry formation of human colorectal cancer through HIF-1α and epithelial mesenchymal transitionReport as inadecuate




Yi Ai Fang, a traditional Chinese herbal formula, impacts the vasculogenic mimicry formation of human colorectal cancer through HIF-1α and epithelial mesenchymal transition - Download this document for free, or read online. Document in PDF available to download.

BMC Complementary and Alternative Medicine

, 16:428

Basic research

Abstract

BackgroundYi Ai Fang YAF, a traditional Chinese medicine TCM formula, has been identified to have anticancer activity in our previously studies. The present study aimed to explore the potential mechanism of YAF suppression of VM on colorectal cancer CRC in vitro and in vivo.

MethodsCell viability was measured by CCK-8 assay. HIF-1α, E-cdE-cadherin, Claudin-4, and VIM Vimentin expressions level in vitro were evaluated by Western blot or RT-PCR. In addition, Human CRC HCT-116 cells were implanted in BALB-c nude mice; mice with xenografted tumors were randomly administrated vehicle control, 8, 16, or 32 mg-mL YAF, or 1 mg-mL fluorouracil 5-FU. HIF-1α, E-cd, Claudin-4, and VIM expression in these tumors were determined by IHC.

ResultsYAF effectively inhibited the growth and the formation of vasculogenic mimicry VM of CRC cells in a dose-dependent trend. YAF restrained the formation of vasculogenic mimicryVM through HIF-1α-EMT pathway in CRC. YAF suppressed VM was triggered by activation of E-cd and Claudin-4,which are characteristics of endothelial cells,and inhibition of HIF-1α and VIM in vitro. In vivo data showed that YAF remarkably inhibited growth of the xenografted tumors. The YAF-treated tumor samples were analyzed by IHC for levels of HIF-1α-EMT related proteins HIF-1α, E-cd, Claudin-4, and VIM. The results indicated that YAF significantly enhanced expression of E-cd and Claudin-4,but decreased expression of HIF-1α, VIM in a dose-dependent manner.

ConclusionsIn conclusion, this study provided the first direct evidence that YAF inhibited the formation of VM in human CRC, suggesting that YAF may be considered as a useful target for cancer therapy.

KeywordsYi Ai Fang HIF-1α EMT Colorectal cancer Vasculogenic mimicry  Download fulltext PDF



Author: Fenggang Hou - Wen Li - Qi Shi - Hongjia Li - Shanshan Liu - Shaoqi Zong - Jianlin Ren - Jie Chai - Jian Xu

Source: https://link.springer.com/







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