Endogenous ABA content in relation to maturation of somatic embryos in Tulipa L. ‘Apeldoorn’ culturesReport as inadecuate




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Acta Physiologiae Plantarum

, 38:270

First Online: 02 November 2016Received: 28 April 2016Revised: 06 July 2016Accepted: 22 October 2016

Abstract

The aim of the present study was to estimate the endogenous abscisic acid ABA content in tulip ‘Apeldoorn’ torpedo and mature somatic embryos. Moreover, the effect of exogenous ABA and-or its inhibitor fluridone on somatic embryo maturation and conversion into plantlets was investigated. Torpedo-stage somatic embryos were subcultured on media containing 5 μM of picloram and 1 μM of 6-benzyl-aminopurine BAP—control, and combinations of ABA 0 or 10 μM and-or fluridone 0 or 30 μM for 1 week. Then, the torpedo embryos were transferred to a maturation medium containing 0.25 μM of α-naphthaleneacetic acid NAA and 2.5 μM of BAP, without ABA and fluridone treatment, and cultivated under darkness or light for ten weeks. Endogenous ABA content first time measured in tulip somatic embryos was evaluated by ELISA test. The obtained results revealed that the highest level of endogenous ABA, at 17.45 nmol g dry weight DW, was recorded in torpedo-stage of tulip embryo development, only after 1 week of ABA treatment, and was nearly 10 times higher in comparison with the control. Simultaneous addition of ABA and fluridone to the medium resulted in the lowering of the ABA concentration to 9.58 nmol g DW. During ten weeks of maturation of the embryos, the endogenous ABA content in mature tissue of tulip somatic embryo considerably decreased to an amount 0.87–1.33 nmol g DW irrespective of ABA and fluridone treatment and did not differ significantly from control 0.59 nmol g DW. Exogenous ABA and fluridone significantly decreased the growth value of fresh weight FW of the tulip torpedo-shaped and mature embryos under light conditions. Percentage of the DW of the torpedo embryos treated with exogenous ABA was significantly higher 15.43–17.02 in comparison with the control 10.87. Three to three and a half times more malformed mature embryos were noted under light conditions than in darkness, irrespective of ABA and fluridone treatment. The highest percentage of mature embryos forming shoots conversion was observed under light conditions in the control and after fluridone treatment 26 and 20%, respectively.

KeywordsTulipa Somatic embryo maturation ABA Fluridone Communicated by J van Huylenbroeck.

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Author: Małgorzata Maślanka - Anna Bach - Franciszek Janowiak

Source: https://link.springer.com/







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