A proteomic analysis of acute leukemia cells treated with 9-hydroxyoctadecadienoic acidReport as inadecuate




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Lipids in Health and Disease

, 15:192

First Online: 10 November 2016Received: 17 November 2015Accepted: 01 November 2016

Abstract

Background9s-hydroxy-octadecadienoic acid 9S-HOD, one of the natural products of linoleic acid oxygenation by 15-lipoxygenase 15-LOX, has been found to have anti-tumor properties in vitro and in vivo. The present study therefore investigated whether 9S-HOD affects acute leukemia HL-60 cells.

MethodsThe cytotoxicity of 9S-HOD in HL-60 with or without the presence of fetal bovine serum FBS in the culture media was tested using cell viability assays and flow cytometry. To explore the mechanism of its anti-tumor activity by 9S-HOD, we used a proteomic analysis to identify HL-60 cells protein profiles, based on two-dimensional gel electrophoresis 2-DE and mass spectrometry MS identification.

Results9S-HOD exerted cytotoxicity efficacy and induced apoptosis in HL-60 cells, and the cytotoxicity was largely attenuated by the presence of FBS in culture media. The proteomic results revealed that 9S-HOD remarkably altered the abundance of 23 proteins that were involved in mRNA metabolic process, protein binding, DNA replication and apoptosis.

ConclusionsOur results indicated that 9S-HOD exerts cytotoxicity in HL-60 cells by affecting several pathways.

Keywords9s-hydroxy-octadecadienoic acid Acute leukemia cells Proteomics Bioinformatics analysis Abbreviations13-HOD13-hydroxy-octadecadienoic acid

15-LOX15-lipoxygenase

2-DETwo-dimensional gel electrophoresis

9-HOD9-hydroxy-octadecadienoic acid

9S-HOD9s-hydroxy-octadecadienoic acid

AMLAcute myeloid leukemia

DAPIDihydrochloride

FBSFetal bovine serum

HODEsHydroxyl-octadecadienoic acids

MSMass spectrometry

PIPropidium iodide

PMFsPeptide mass fingerprints

t10,c12-CLAt10,c12-Conjugated linoleic acid

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Author: Zhen Li - Bohong Chen - Ping Wang - Xin Li - Gaotai Cai - Wei Wei - Wenqi Dong

Source: https://link.springer.com/



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