miR-10a inhibits cell proliferation and promotes cell apoptosis by targeting BCL6 in diffuse large B-cell lymphomaReport as inadecuate




miR-10a inhibits cell proliferation and promotes cell apoptosis by targeting BCL6 in diffuse large B-cell lymphoma - Download this document for free, or read online. Document in PDF available to download.

Protein and Cell

, Volume 7, Issue 12, pp 899–912

First Online: 04 November 2016Received: 08 July 2016Accepted: 06 August 2016

Abstract

The BCL6 B-Cell Lymphoma 6 gene is a proto-oncogene that is often expressed in diffuse large B-cell lymphomas DLBCLs. BCL6 loss of function can kill DLBCL cells, demonstrating that BCL6 is necessary for the survival of DLBCL cells and could be a therapeutic target. In this study, we found that BCL6 protein levels were consistently upregulated in DLBCL tissues, whereas its mRNA levels varied randomly in tissues, suggesting that a post-transcriptional mechanism was involved in BCL6 regulation. We used bioinformatics analysis to search for miRNAs, which potentially target BCL6, and identified specific targeting sites for miR-10a in the 3′-untranslated region 3′-UTR of BCL6. We further identified an inverse correlation between miR-10a levels and BCL6 protein levels, but not mRNA levels, in DLBCL tumor tissue samples. By overexpressing or knocking down miR-10a in DLBCL cells, we experimentally validated that miR-10a directly recognizes the 3′-UTR of the BCL6 transcript and regulated BCL6 expression. Furthermore, we demonstrated that negatively regulating BCL6 by miR-10a suppressed the proliferation and promoted apoptosis of DLBCL cells.

KeywordsmicroRNA miR-10a BCL6 DLBCL proliferation apoptosis Qian Fan, Xiangrui Meng, Hongwei Liang, and Huilai Zhang have contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1007-s13238-016-0316-z contains supplementary material, which is available to authorized users.

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Author: Qian Fan - Xiangrui Meng - Hongwei Liang - Huilai Zhang - Xianming Liu - Lanfang Li - Wei Li - Wu Sun - Haiyang Zhang - K

Source: https://link.springer.com/







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