The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathwayReport as inadecuate




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BMC Complementary and Alternative Medicine

, 16:514

Basic research

Abstract

BackgroundDanQi pill DQP is prescribed widely in China and has definite cardioprotective effect on coronary heart disease. Our previous studies proved that DQP could effectively regulate plasma levels of high density lipoprotein HDL and low density lipoprotein LDL. However, the regulatory mechanisms of DQP and its major components Salvianolic acids and Panax notoginseng saponins DS on lipid metabolism disorders haven’t been comprehensively studied so far.

MethodsRat model of coronary heart disease was induced by left anterior descending LAD artery ligation operations. Rats were divided into sham, model, DQP treated, DS treated and positive drug clofibrate treated groups. At 28 days after surgery, cardiac functions were assessed by echocardiography. Expressions of transcription factors and key molecules in energy metabolism pathway were measured by reverse transcriptase polymerase chain reaction or western blotting.

ResultsIn ischemic heart model, cardiac functions were severely injured but improved by treatments of DQP and DS. Expression of LPL was down-regulated in model group. Both DQP and DS could up-regulate the mRNA expression of LPL. Membrane proteins involved in lipid transport and uptake, such as FABP4 and CPT-1A, were down-regulated in ischemic heart tissues. Treatment with DQP and DS regulated lipid metabolisms by up-regulating expressions of FABP4 and CPT-1A. DQP and DS also suppressed expression of cytochrome P450. Furthermore, transcriptional factors, such as PPARα, PPARγ, RXRA and PGC-1α, were down-regulated in ischemic model group. DQP and DS could up-regulate expressions of these factors. However, DS showed a better efficacy than DQP on PGC-1α, a coactivator of PPARs. Key molecules in signaling pathways such as AKT1-2, ERK and PI3K were also regulated by DQP and DS simultaneously.

ConclusionsSalvianolic acids and Panax notoginseng are the major effective components of DanQi pill in improving lipid metabolism in ischemic heart model. The effects may be mediated by regulating transcriptional factors such as PPARs, RXRA and PGC-1α.

KeywordsComponents Coronary heart disease PPARs-PGC1α pathway DanQi pill AbbreviationsABCA1ATP-binding cassette transporter

ApoA-IApolipoprotein A-I

CD36Cluster of differentiation 36

CHDCoronary heart disease

CPT-1ACarnitine palmitoyltransferase 1A

DQPDanQi pill

DSPanax notoginseng saponins

EFEjection fraction

ERK1-2Extracellular signal-regulated kinase

FABPFatty acid binding protein

FSFractional shortening

GAPDHGlyceraldehyde-3-phosphate dehydrogenase

HDLHigh density lipoprotein

HMGCR3-hydroxy-3methyl-glutaryl-CoA reductase

LADLeft anterior descending

LCATLecithin cholesterolacyltransferase

LDLLow density lipoprotein

LEVSvLeft ventricular end-systolic volume

LVEDdLeft ventricular end-diastolic diameter

LVEDvLeft ventricular end-diastolic volume

LVESdLeft ventricular end-systolic diameter

MAPKMitogen-activated protein kinase

PGC-1αPeroxisome proliferator-activated receptor gamma coactivator-1α

PI3KPhosphatidylinositol 3-kinase

PNSPanax Notoginseng Saponins

PPARPeroxisome proliferator-activated receptor family

RT-PCRReverse transcriptase polymerase chain reaction

RXRARetinoic acid receptor alfa

SASalvianolic acid

SDSSodium dodecyl sulphate

TCMTraditional Chinese medicine

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Author: Qiyan Wang - Chun Li - Qian Zhang - Yuanyuan Wang - Tianjiao Shi - Linghui Lu - Yi Zhang - Yong Wang - Wei Wang

Source: https://link.springer.com/



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