Chungsimyeonja-eum inhibits inflammatory responses in RAW 264.7 macrophages and HaCaT keratinocytesReport as inadecuate




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BMC Complementary and Alternative Medicine

, 15:371

First Online: 16 October 2015Received: 07 July 2015Accepted: 07 October 2015

Abstract

BackgroundChungsimyeonja-eum CSYJE is an herbal prescription used in traditional Oriental medicine for treating cerebral infarction by reducing ischemic damage. However, the effects of CSYJE on inflammation have not been verified scientifically.

MethodsAnti-inflammatory effects of CSYJE was investigated to dertermine the inhibitory effects of CSYJE against inflammation using RAW 264.7 mouse macrophages and HaCaT human keratinocytes. To measure the effects of CSYJE on inflammatory mediators and cytokines-chemokines, we used the following methods: cell viability assay, enzyme-linked immunosorbent assay ELISA, western blotting, immunocytochemistry. RAW 264.7 cells were pretreated with CSYJE 250, 500, or 1000 μg-mL for 4 h and treated with lipopolysaccharide LPS for additional 20 h. HaCaT cells were stimulated with tumor necrosis factor alpha TNF-α and interferon gamma IFN-γ TI, and CSYJE 125, 250, or 500 μg-mL for 24 h.

ResultsCSYJE suppressed the production of nitric oxide NO, IC50 1000 μg-mL, prostaglandin E2 PGE2, IC50 = 12.1 μg-mL, and interleukin IL-6 IC50 = 248 μg-mL in LPS-stimulated RAW 264.7 cells. CSYJE suppressed the effects of TI on the production of thymus and activation-regulated chemokine TARC, IC50 = 330.2 μg-mL, macrophage-derived chemokine MDC-CCL22, IC50 = 52.5 μg-mL, regulated on activation, normal T-cell expressed and secreted RANTES-CCL5, IC50 = 372.9 μg-mL, and IL-8 IC50 = 345.1 μg-mL in HaCaT cells. CSYJE inhibited TI-stimulated STAT1 phosphorylation in a dose-dependent manner and nuclear translocation at 500 μg-mL in HaCaT cells.

ConclusionOur results suggest a possible therapeutic application of CSYJE for treating inflammatory diseases.

KeywordsChungsimyeonja-eum Inflammation Cytokine Chemokine Macrophage Keratinocyte  Download fulltext PDF



Author: Hye-Sun Lim - Kim Yeji - Chang-Seob Seo - Sae-Rom Yoo - Seong-Eun Jin - Hyeun-Kyoo Shin - Soo-Jin Jeong

Source: https://link.springer.com/



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