Fluorescence-tagged metallothionein with CdTe quantum dots analyzed by the chip-CE techniqueReport as inadecuate




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Journal of Nanoparticle Research

, 17:423

First Online: 28 October 2015Received: 09 July 2015Accepted: 15 October 2015

AbstractQuantum dots QDs are fluorescence nanoparticles NPs with unique optic properties which allow their use as probes in chemical, biological, immunological, and molecular imaging. QDs linked with target ligands such as peptides or small molecules can be used as tumor biomarkers. These particles are a promising tool for selective, fast, and sensitive tagging and imaging in medicine. In this study, an attempt was made to use QDs as a marker for human metallothionein MT isoforms 1 and 2. Four kinds of CdTe QDs of different sizes bioconjugated with MT were analyzed using the chip-CE technique. Based on the results, it can be concluded that MT is willing to interact with QDs, and the chip-CE technique enables the observation of their complexes. It was also observed that changes ranging roughly 6–7 kDa, a value corresponding to the MT monomer, depend on the hydrodynamic diameters of QDs; also, the MT sample without cadmium interacted stronger with QDs than MT saturated with cadmium. Results show that MT is willing to interact with smaller QDs blue CdTe rather than larger ones QDs red CdTe. To our knowledge, chip-CE has not previously been applied in the study of CdTe QDs interaction with MT.

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AbstractOpen image in new windowKeywordsBioconjugation Chip-CE Metallothionein Quantum dots Biomarkers Health effects AbbreviationsCECapillary electrophoresis

LDSLithium dodecyl sulfate

MrMolecular weight

MTMetallothionein

NPsNanoparticles

QDsQuantum dots

Electronic supplementary materialThe online version of this article doi:10.1007-s11051-015-3226-8 contains supplementary material, which is available to authorized users.

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Author: Ewelina Guszpit - Sona Krizkova - Marta Kepinska - Miguel Angel Merlos Rodrigo - Halina Milnerowicz - Pavel Kopel - Rene 

Source: https://link.springer.com/



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