Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic SeleniumReport as inadecuate




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Bulletin of Environmental Contamination and Toxicology

, Volume 95, Issue 5, pp 593–598

First Online: 23 July 2015Received: 12 February 2015Accepted: 15 July 2015

Abstract

The effect of selenium applied as sodium selenite Na2SeO3 on the cytotoxicity of vanadyl sulphate VOSO4 was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na2SeO3 at low doses 0.5 and 1 μM can enhance 100 μM VOSO4-induced cell damage. The two-way ANOVA analysis revealed that the increased cell damage was a consequence of a synergistic interaction of 0.5 μM Na2SeO3 with VOSO4 and 1 μM Na2SeO3 with VOSO4. Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO4 alone. In turn, a majority of cells co-treated with VOSO4 and 1 μM Na2SeO3 were elongated, and exhibited cytoplasmic vacuolization. These results warn of the potential contribution of inorganic selenium to vanadium-induced toxicity.

KeywordsSelenium Vanadium Interaction Cytotoxicity CHO-K1 cells  Download fulltext PDF



Author: Iwona Zwolak

Source: https://link.springer.com/



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