The potential of bacteriophage cocktail in eliminating Methicillin-resistant Staphylococcus aureus biofilms in terms of different extracellular matrices expressed by PIA, ciaA-D and FnBPA genesReport as inadecuate




The potential of bacteriophage cocktail in eliminating Methicillin-resistant Staphylococcus aureus biofilms in terms of different extracellular matrices expressed by PIA, ciaA-D and FnBPA genes - Download this document for free, or read online. Document in PDF available to download.

Annals of Clinical Microbiology and Antimicrobials

, 14:49

First Online: 11 November 2015Received: 14 February 2015Accepted: 05 October 2015

Abstract

BackgroundThis study assessed novel approach of using highly lytic phages against methicillin-susceptible Staphylococcus aureus MSSA and methicillin-resistant Staphylococcus aureus MRSA biofilms with and without biofilm extracellular matrix- disrupting chemical.

MethodThe resultant phage-based control was assessed in relation to the type of biofilm extracellular matrix namely, polysaccharide intercellular adhesion PIA or proteinacious fibronectin-binding protein A FnBPA. The biofilms were formed in vitro by 24 h incubation of bacteria in 96 wells microtiter plates at room temperature. The formed biofilms were assessed by tissue culture plate TCP. Moreover, the nature of the biofilm was assessed by scanning electron microscopy SEM and PCR assay for detecting PIA genes, ciaA-D and FnBPA genes.

Resultsthis study showed that applied phages with 0.08 % benezenthonium chloride, for PIA biofilms, and 0.06 % ethanol, for proteinacious FnBPA biofilms, exerted 100 % eradication for MSSA biofilms and about 78 % of MRSA biofilms. The phage-based control of biofilms with chemical adjuvant showed significantly higher efficiency than that without adjuvant P < 0.05. Moreover, FnBPA biofilms were more common in MRSA than in MSSA while PIA biofilms were more common in MSSA than in MRSA. And the most resistant type of biofilms to phage-based control was FnBPA in MRSA where 50 % of biofilms were reduced but not eradicated completely.

ConclusionsIt is concluded that PIA-disturbing agent and protein denaturing alcohol can increase the efficiency of attacking phages in accessing host cell walls and lysing them which in turn lead to much more efficient MRSA and MSSA biofilm treatment and prevention.

KeywordsMethicillin-resistant Staphylococcus aureus MRSA MSSA Biofilm Bacteriophages PIA FnBPA  Download fulltext PDF



Author: Ahmed Sahib Abdulamir - Sabah A. A. Jassim - Rand R. Hafidh - Fatimah Abu Bakar

Source: https://link.springer.com/







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