Molecular breeding of lignin-degrading brown-rot fungus Gloeophyllum trabeum by homologous expression of laccase geneReport as inadecuate




Molecular breeding of lignin-degrading brown-rot fungus Gloeophyllum trabeum by homologous expression of laccase gene - Download this document for free, or read online. Document in PDF available to download.

AMB Express

, 5:81

First Online: 22 December 2015Received: 16 September 2015Accepted: 11 December 2015

Abstract

The basidiomycete Gloeophyllum trabeum KU-41 can degrade Japanese cedar wood efficiently. To construct a strain better suited for biofuel production from Japanese cedar wood, we developed a gene transformation system for G. trabeum KU-41 using the hygromycin phosphotransferase-encoding gene hpt as a marker. The endogenous laccase candidate gene Gtlcc3 was fused with the promoter of the G. trabeum glyceraldehyde-3-phosphate dehydrogenase-encoding gene and co-transformed with the hpt-bearing pAH marker plasmid. We obtained 44 co-transformants, and identified co-transformant L#61, which showed the highest laccase activity among all the transformants. Moreover, strain L#61 was able to degrade lignin in Japanese cedar wood-containing medium, in contrast to wild-type G. trabeum KU-41 and to a typical white-rot fungus Phanerochaete chrysosporium. By using strain L#61, direct ethanol production from Japanese cedar wood was improved compared to wild type. To our knowledge, this study is the first report of the molecular breeding of lignin-degrading brown-rot fungus and direct ethanol production from softwoods by co-transformation with laccase overproduction constructs.

KeywordsGloeophyllum trabeum KU-41 Laccase gene Homologous expression Lignin degradation Electronic supplementary materialThe online version of this article doi:10.1186-s13568-015-0173-9 contains supplementary material, which is available to authorized users.

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Author: Misa Arimoto - Kenji Yamagishi - Jianqiao Wang - Kanade Tanaka - Takanori Miyoshi - Ichiro Kamei - Ryuichiro Kondo - Toshio

Source: https://link.springer.com/







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