The influence of aminophylline on the nanostructure and nanomechanics of T lymphocytes: an AFM studyReport as inadecuate




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Nanoscale Research Letters

, 9:518

Nanotechnology and Nanomaterials NANO-2013

Abstract

Although much progress has been made in the illustration of the mechanism of aminophylline AM treating asthma, there is no data about its effect on the nanostructure and nanomechanics of T lymphocytes. Here, we presented atomic force spectroscopy AFM-based investigations at the nanoscale level to address the above fundamental biophysical questions. As increasing AM treatment time, T lymphocytes- volume nearly double increased and then decreased. The changes of nanostructural features of the cell membrane, i.e., mean height of particles, root-mean-square roughness Rq, crack and fragment appearance, increased with AM treatment time. T lymphocytes were completely destroyed with 96-h treatment, and they existed in the form of small fragments. Analysis of force-distance curves showed that the adhesion force of cell surface decreased significantly with the increase of AM treatment time, while the cell stiffness increased firstly and then decreased. These changes were closely correlated to the characteristics and process of cell oncosis. In total, these quantitative and qualitative changes of T lymphocytes- structure and nanomechanical properties suggested that AM could induce T lymphocyte oncosis to exert anti-inflammatory effects for treating asthma. These findings provide new insights into the T lymphocyte oncosis and the anti-inflammatory mechanism and immune regulation actions of AM.

KeywordsAtomic force microscopy T lymphocytes Aminophylline Nanostructure Nanomechanics AbbreviationsAFMatomic force spectroscopy

AMaminophylline

FWHMfull width at half maximum

Rqroot-mean-square roughness.

Electronic supplementary materialThe online version of this article doi:10.1186-1556-276X-9-518 contains supplementary material, which is available to authorized users.

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Author: Xun Huang - Jiexiang He - Mingxian Liu - Changren Zhou

Source: https://link.springer.com/







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