Stepwise enhancement of catalytic performance of haloalkane dehalogenase LinB towards β-hexachlorocyclohexaneReport as inadecuate




Stepwise enhancement of catalytic performance of haloalkane dehalogenase LinB towards β-hexachlorocyclohexane - Download this document for free, or read online. Document in PDF available to download.

AMB Express

, 4:72

First Online: 21 September 2014Received: 11 July 2014Accepted: 18 August 2014

Abstract

Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid residues, exhibit only seven amino acid residue differences between them, but LinBMI’s catalytic performance towards β-hexachlorocyclohexane β-HCH is considerably higher than LinBUT’s. To elucidate the molecular basis governing this difference, intermediate mutants between LinBUT and LinBMI were constructed and kinetically characterized. The activities of LinBUT-based mutants gradually increased by cumulative mutations into LinBUT, and the effects of the individual amino acid substitutions depended on combination with other mutations. These results indicated that LinBUT’s β-HCH degradation activity can be enhanced in a stepwise manner by the accumulation of point mutations.

Keywordsβ-Hexachlorocyclohexane Xenobiotics Biodegradation Haloalkane dehalogenase Protein evolution Electronic supplementary materialThe online version of this article doi:10.1186-s13568-014-0072-5 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Ryota Moriuchi - Hiroki Tanaka - Yuki Nikawadori - Mayuko Ishitsuka - Michihiro Ito - Yoshiyuki Ohtsubo - Masataka Tsuda -

Source: https://link.springer.com/







Related documents