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Burns and Trauma

, Volume 2, Issue 4, pp 181–186

First Online: 25 October 2014Received: 05 July 2014Revised: 09 September 2014Accepted: 15 September 2014

Abstract

Mesenchymal stem cells MSCs have been accepted as a promising cell source in tissue repair and regeneration. However, the inability to enrich MSCs in target areas limits their wide application. As a result, it has been a major goal to induce MSCs to be abundantly and specifically recruited to the injury site. In this study, a peptide with a specific affinity for MSCs E7 peptide was immobilized to a collagen scaffold via a collagen-binding domain CBD to construct a functional collagen scaffold. In addition, the hypothesis that this method could recruit MSCs specifically was evaluated in a porcine model. In vivo investigations indicated that due to the immunore-action, the CBD-MSC-peptide collagen scaffold enhanced MSC adhesion and infiltration and promoted wound healing. At day 7 after surgery, we found more infiltrating cells and capillaries in the Collagen-CBD-E7 peptide group compared to the Scaffold group. At day 14, 21 and 28, a faster healing process was observed in the Collagen-CBD-E7 peptide group, with significant differences compared with the other groups P < 0.05, P < 0.01. The results demonstrate the potential use of targeted therapy to rapidly heal skin wounds.

Key wordsMesenchymal stem cells skin wound healing Collagen-binding domain mesenchymal stem cells affinity peptide How to cite this article : Wang H, Yan X, Shen L, Li S, Lin Y, Wang S, et al. Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs. Burn Trauma 2014;2:181–6.

Source of Support : Supported by the National Nature Science Foundation of China Grants No. 81272108. Conflict of Interest : None declared.

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Author: Huili Wang - Xin Yan - Liangyun Shen - Shiyan Li - Yue Lin - Shuqin Wang - Xiang Lin Hou - Chunying Shi - Yun Yang - Jia

Source: https://link.springer.com/



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