The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat modelReport as inadecuate




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BMC Complementary and Alternative Medicine

, 14:386

Basic research

Abstract

BackgroundGrowing evidence indicates that intestinal microbiota regulate our metabolism. Probiotics confer health benefits that may depend on their ability to affect the gut microbiota. The objective of this study was to examine the effect of supplementation with the probiotic strain, Lactobacillus rhamnosus hsryfm 1301, on the gut microbiota in a hyperlipidemic rat model, and to explore the associations between the gut microbiota and the serum lipids.

MethodsThe hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 d. The rats’ gut microbiota were analyzed using high-throughput sequencing before and after L. rhamnosus hsryfm 1301 supplementation or its fermented milk for 28 d. The serum lipids level was also tested.

ResultsThe rats’ primary gut microbiota were composed of Bacteroidetes, Firmicutes, Proteobacteria, Spirochaetes and Verrucomicrobia. The abundance and diversity of the gut microbiota generally decreased after feeding with a high-fat diet, with a significant decrease in the relative abundance of Bacteroidetes, but with an increase in that of Firmicutes P < 0.05. Administration of L. rhamnosus hsryfm 1301 or its fermented milk for 28 d, could recover the Bacteroidetes and Verrucomicrobia abundance and could decrease the Firmicutes abundance, which was associated with a significant reduction in the serum lipids’ level in the hyperlipidemic rats with high-fat diet induced. The abundance of 22 genera of gut bacteria was changed significantly after probiotic intervention for 28 d P < 0.05. A positive correlation was observed between Ruminococcus spp. and serum triglycerides, Dorea spp. and serum cholesterol TC and low-density lipoprotein LDL-C, and Enterococcus spp. and high-density lipoprotein. The Butyrivibrio spp. negatively correlated with TC and LDL-C.

ConclusionsOur results suggest that the lipid metabolism of hyperlipidemic rats was improved by regulating the gut microbiota with supplementation of L.rhamnosus hsryfm 1301 or its fermented milk for 28 d.

AbbreviationsWHOWorld Health Organization

CFUColony-Forming Units.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-14-386 contains supplementary material, which is available to authorized users.

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Author: Dawei Chen - Zhenquan Yang - Xia Chen - Yujun Huang - Boxing Yin - Feixiang Guo - Haiqing Zhao - Tangyan Zhao - Henxian Q

Source: https://link.springer.com/



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