Epidemiology of human parechovirus, Aichi virus and salivirus in fecal samples from hospitalized children with gastroenteritis in Hong KongReport as inadecuate




Epidemiology of human parechovirus, Aichi virus and salivirus in fecal samples from hospitalized children with gastroenteritis in Hong Kong - Download this document for free, or read online. Document in PDF available to download.

Virology Journal

, 11:182

Positive-strand RNA viruses

Abstract

BackgroundEmerging human picornaviruses, including human parechovirus HPeV, Aichi virus AiV and salivirus SalV were found to be associated with gastroenteritis, but their roles in enteric infections are not fully understood. In addition, no report on the circulation of these viruses in Hong Kong is available. The objective of this study was to investigate the prevalence and genetic diversity of HPeV, AiV and SalV in fecal samples from hospitalized children with gastroenteritis in Hong Kong.

MethodsFecal samples from hospitalized children with gastroenteritis were subject to detection of HPeV, AiV and SalV by RT-PCR using consensus primers targeted to their 5′UTRs. Positive samples were subject to capsid and-or 3CD region analysis for genotype determination. The epidemiology of HPeV, AiV and SalV infections was analyzed.

ResultsAmong 1,708 fecal samples subjected to RT-PCR using primers targeted to 5′UTR of HPeV, AiV and SalV, viruses were detected in 55 samples, with 50 positive for HPeV only, 3 positive for AiV only, 1 positive for both HPeV and AiV, and 1 positive for both HPeV and SalV. Phylogenetic analysis of the partial VP1 gene of the 33 HPeV strains revealed the presence of genotypes of HPeV- 1, 3, 4, 5, 7, 10, among which HPeV-1 was the predominant genotype circulating in our population. The peak activity of HPeV infection was in fall. Of the 3 children with AiV infection, the 3 AiV strains were found to belong to genotype A based on the phylogenetic analysis of their partial VP1 and 3CD regions. The genotype of a SalV strain detected in this study could not be determined. Co-detection of different pathogens was observed in 24 samples 43.6% of 55 fecal samples positive for HPeV, AiV and SalV.

ConclusionsHPeV, AiV and SalV were detected in fecal samples of hospitalized children with gastroenteritis in Hong Kong, with the former having the highest prevalence. HPeV-1 was the predominant genotype among HPeVs, while genotype A was the predominant genotype among AiVs in this study.

KeywordsHuman parechovirus Aichi virus Salivirus Gastroenteritis Fecal children Hong Kong AbbreviationsHPeVHuman parechovirus

AiVAichi virus

SalVSalivirus

RT-PCRReverse transcription polymerase chain reaction

UTRUntranslated region

HBoVHuman bocavirus

HKHong Kong.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-11-182 contains supplementary material, which is available to authorized users.

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Author: Cyril C Y Yip - Kin-Land Lo - Tak-Lun Que - Rodney A Lee - Kwok-Hung Chan - Kwok-Yung Yuen - Patrick C Y Woo - Susanna

Source: https://link.springer.com/







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