Complete genome sequence of a broad-host-range lytic Dickeya spp. bacteriophage ϕD5Report as inadecuate




Complete genome sequence of a broad-host-range lytic Dickeya spp. bacteriophage ϕD5 - Download this document for free, or read online. Document in PDF available to download.

Archives of Virology

, Volume 159, Issue 11, pp 3153–3155

First Online: 08 July 2014Received: 17 April 2014Accepted: 29 June 2014

Abstract

Little is known about lytic bacteriophages infecting plant-pathogenic Dickeya spp. These bacteria cause economically significant losses in arable crops and ornamental plant production worldwide. At present, there is no effective control of diseases caused by Dickeya spp. A novel bacteriophage, ϕD5, belonging to the family Myoviridae, order Caudovirales, that could be used to control these bacteria was isolated previously. This report provides information on its characterization. The ϕD5 genome consists of 155,346-bp-length double-stranded DNA with a GC content of 49.7 % and is predicted to have 196 open reading frames ORFs with an average length of 711 nucleotides each. The ORFs were classified into functional groups, including phage structure, packaging, DNA metabolism, regulation, and additional functions. The phage lifestyle predicted from PHACTS indicated that ϕD5 may be a lytic phage and therefore can efficiently kill plant-pathogenic Dickeya spp.

The nucleotide sequence accession numberThe complete genome sequence of bacteriophage ϕD5 is available in the GenBank https:-www.ncbi.nlm.nih.gov-genbank- database under accession number KJ716335.

Electronic supplementary materialThe online version of this article doi:10.1007-s00705-014-2170-8 contains supplementary material, which is available to authorized users.

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Author: Robert Czajkowski - Zofia Ozymko - Szymon Zwirowski - Ewa Lojkowska

Source: https://link.springer.com/







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