Classical swine fever virus induces oxidative stress in swine umbilical vein endothelial cellsReport as inadecuate




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BMC Veterinary Research

, 10:279

Virology

Abstract

BackgroundClassical swine fever virus CSFV infection causes significant losses of pigs, which is characterized by hemorrhage, disseminated intravascular coagulation and leucopenia. The swine vascular endothelial cell is a primary target cell for CSFV. The aim of this study was to determine the role of CSFV infection in inducing oxidative stress OS in vascular endothelial cells.

ResultsWe demonstrated that CSFV infection induced oxidative stress in swine umbilical vein endothelial cells SUVECs, characterized by the induction of reactive oxygen species ROS production and the elevations of porcine antioxidant proteins thioredoxin Trx, peroxiredoxin-6 PRDX-6 and heme oxygenase-1 HO-1 expression. Furthermore, cyclooxygenase-2 COX-2, a pro-inflammatory protein related to oxidative stress, was up-regulated while anti-inflammatory protein peroxisome proliferator-activated receptor-γ PPAR-γ, an important mediator in vascular functional regulation, was down-regulated in the CSFV infected cells. In addition, antioxidants showed significant inhibitory effects on the CSFV replication, indicating a close relationship between CSFV replication and OS induced in the host cells.

ConclusionsOur results indicated that CSFV infection induced oxidative stress in SUVECs. These findings provide novel information on the mechanism by which CSFV can alter intracellular events associated with the viral infection.

KeywordsClassical swine fever virus Oxidative stress Antioxidant protein Reactive oxygen species Inflammatory response AbbreviationsCSFVClassical swine fever virus

OSOxidative stress

SUVECsSwine umbilical vein endothelial cells

VECsVascular endothelial cells

ROSReactive oxygen species

TrxThioredoxin

PRDX-6Peroxiredoxin-6

HO-1Heme oxygenase-1

COX-2Cyclooxygenase-2

PPAR-γPeroxisome proliferator-activated receptor-γ

NONitric oxide

GSHGlutathione

NACN-acetyl-L-cysteine

BHAButylated hydroxyanisole

ASAAspirin

FCSFetal calf serum

HBSSHank’s balanced salt solution

Electronic supplementary materialThe online version of this article doi:10.1186-s12917-014-0279-3 contains supplementary material, which is available to authorized users.

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Author: Lei He - Yanming Zhang - Yanqin Fang - Wulong Liang - Jihui Lin - Min Cheng

Source: https://link.springer.com/



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