The AtRAD21.1 and AtRAD21.3 Arabidopsis cohesins play a synergistic role in somatic DNA double strand break damage repairReport as inadecuate




The AtRAD21.1 and AtRAD21.3 Arabidopsis cohesins play a synergistic role in somatic DNA double strand break damage repair - Download this document for free, or read online. Document in PDF available to download.

BMC Plant Biology

, 14:353

Development and cell biology

Abstract

BackgroundThe RAD21 cohesin plays, besides its well-recognised role in chromatid cohesion, a role in DNA double strand break dsb repair. In Arabidopsis there are three RAD21 paralog genes AtRAD21.1, AtRAD21.2 and AtRAD21.3, yet only AtRAD21.1 has been shown to be required for DNA dsb damage repair. Further investigation of the role of cohesins in DNA dsb repair was carried out and is here reported.

ResultsWe show for the first time that not only AtRAD21.1 but also AtRAD21.3 play a role in somatic DNA dsb repair. Comet data shows that the lack of either cohesins induces a similar high basal level of DNA dsb in the nuclei and a slower DNA dsb repair kinetics in both cohesin mutants. The observed AtRAD21.3 transcriptional response to DNA dsb induction reinforces further the role of this cohesin in DNA dsb repair. The importance of AtRAD21.3 in DNA dsb damage repair, after exposure to DNA dsb damage inducing agents, is notorious and recognisably evident at the phenotypical level, particularly when the AtRAD21.1 gene is also disrupted.

Data on the kinetics of DNA dsb damage repair and DNA damage sensitivity assays, of single and double atrad21 mutants, as well as the transcription dynamics of the AtRAD21 cohesins over a period of 48 hours after the induction of DNA dsb damage is also shown.

ConclusionsOur data demonstrates that both Arabidopsis cohesin AtRAD21.1 and AtRAD21.3 play a role in somatic DNA dsb repair. Furthermore, the phenotypical data from the atrad21.1 atrad21.3 double mutant indicates that these two cohesins function synergistically in DNA dsb repair. The implications of this data are discussed.

KeywordsArabidopsis AtRAD21.1 AtRAD21.3 Cohesins Comet assay DNA damage Gene expression AbbreviationsAEIAfter end of irradiation exposure

C-NHEJCanonical non-homologous end-joining DNA repair pathway

ColWild-type Columbia-0

CompComplementation lines

dsbDouble strand break

GMGermination solid medium

HRHomologous recombination DNA repair pathway

NHEJNon-homologous end-joining DNA repair pathway

qRT-PCRQuantitative real-time PCR

RT-PCRReverse transcription PCR

Electronic supplementary materialThe online version of this article doi:10.1186-s12870-014-0353-9 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: José A da Costa-Nunes - Cláudio Capitão - Jaroslav Kozak - Pedro Costa-Nunes - Gloria M Ducasa - Olga Pontes - Karel J

Source: https://link.springer.com/







Related documents