Chemical composition, antinociceptive, anti-inflammatory and redox properties in vitro of the essential oil from Remirea maritima Aubl. CyperaceaeReport as inadecuate




Chemical composition, antinociceptive, anti-inflammatory and redox properties in vitro of the essential oil from Remirea maritima Aubl. Cyperaceae - Download this document for free, or read online. Document in PDF available to download.

BMC Complementary and Alternative Medicine

, 14:514

Basic research

Abstract

BackgroundThe present study was carried out to evaluate antioxidant, antinociceptive and anti-inflammatory activities of essential oil from R. maritima RMO in experimental protocols.

MethodsThe essential oil from the roots and rhizomes of RMO were obtained by hydrodistillation using a Clevenger apparatus, and analyzed by gas chromatography-mass spectrometry GC-MS. Here, we evaluated free radical scavenging activities and antioxidant potential of RMO using in vitro assays for scavenging activity against hydroxyl radicals, hydrogen peroxide, superoxide radicals, and nitric oxide. The total reactive antioxidant potential TRAP and total antioxidant reactivity TAR indexes and in vitro lipoperoxidation were also evaluated. The ability of RMO to prevent lipid peroxidation was measured by quantifying thiobarbituric acid-reactive substances TBARS. NO radical generated at physiological pH was found to be inhibited by RMO, that showed scavenging effect upon SNP-induced NO production at all concentrations. Antinociceptive and anti-inflammatory properties were evaluated by acetic acid writhing reflex, Formalin-induced nociception and Carrageenan-induced edema test.

ResultsThe majors compounds identified was remirol 43.2%, cyperene 13.8%, iso-evodionol 5.8%, cyperotundone 5.7%, caryophyllene oxide 4.9%, and rotundene 4.6%. At the TRAP assay, RMO concentration of 1 mg.mL showed anti-oxidant effects and at concentration of 1 and 10 ng.mL RMO showed pro-oxidant effect. RMO at 1 mg.mL also showed significant anti-oxidant capacity in TAR measurement. Concentrations of RMO from 1 ng.mL to 100 μg.mL enhanced the AAPH-induced lipoperoxidation. RMO reduced deoxyribose oxidative damage, induced by the Fenton reaction induction system, at concentrations from 1 ng.mL to 100 μg.mL. We observed that RMO caused a significant increase in rate of adrenaline auto-oxidation. On the other hand RMO did not present any scavenging effect in H2O2 formation in vitro. The results of this study revealed that RMO has both peripheral and central analgesic properties. The RMO, all doses, orally p.o. administered significantly inhibited p < 0.05, p < 0.01 and p < 0.001 the acetic acid-induced writhings and two phases of formalin-induced nociception in mice.

ConclusionThe RMO demonstrated antioxidant and analgesic profile which may be related to the composition of the oil.

KeywordsRemirea maritima Essential oil composition Redox properties AbbreviationsAAPH2,2-Azobisisobutyramidinium chloride

ANOVAAnalysis of variance

AUCArea under the curve

CATCatalase-like activity

CLChemiluminescense

CNSCentral nervous system

DRDeoxyribose

EDTAEthylenediamine tetraacetic acid

GCGas chromatography

i. p.Intraperitoneal

INDOIndomethacin

MSMass spectrometry

NBTNitroblue tetrazolium

NONitric oxide

p.o.per os

RMOEssential oil from R. maritima

ROSReactive oxygen species

SNPSodium nitroprusside

SODSuperoxide dismutase

TARTotal antioxidant reactivity

TBAThiobarbituric acid

TBARSThiobarbituric acid-reactive substances

TLCThin-layer chromatography

TRAPTotal reactive antioxidant potential.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-14-514 contains supplementary material, which is available to authorized users.

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Author: Alessandra Silva Rabelo - Mairim Russo Serafini - Thallita Kelly Rabelo - Marcelia Garcez Dória de Melo - Douglas da Si

Source: https://link.springer.com/



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