Early changes of the kinetics of monocyte trem-1 reflect final outcome in human sepsisReport as inadecuate




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BMC Immunology

, 15:585

Immunity to infection

Abstract

BackgroundTREM-1 triggering receptor expressed on myeloid cells, a receptor expressed on neutrophils and monocytes, is upregulated in sepsis and seems to tune the inflammatory response. We explored the expression of TREM-1 at the gene level and on cell membranes of monocytes and association with clinical outcome.

MethodsPeripheral venous blood was sampled from 75 septic patients 39 patients with sepsis, 25 with severe sepsis and 11 with septic shock on sepsis days 1, 3 and 7. TREM-1 on monocytes was measured by flow cytometry; gene expression of TREM-1 in circulating mononuclear cells was assessed by real-time PCR. sTREM-1 was measured in serum by an enzyme immunoassay.

ResultsAlthough surface TREM-1, sTREM-1 and TREM-1 gene expression did not differ between sepsis, severe sepsis and septic shock on day 1, survivors had greater expression of surface TREM-1 on days 3 and 7 compared to non-survivors. sTREM-1 on non-survivors decreased on day 3 compared to baseline. Patients with increase of monocyte gene expression of TREM-1 from day 1 to day 3 had prolonged survival compared to patients with decrease of gene expression of TREM-1 from day 1 to day 3 p: 0.031.

ConclusionsEarly decrease of gene expression of TREM-1 in monocytes is associated with poor outcome. A reciprocal decrease of the pro-inflammatory surface receptor TREM-1 linked with sepsis-induced immunosuppression may be part of the explanation.

KeywordsSepsis Outcome TREM-1 sTREM-1, Monocytes AbbreviationsAPACHEAcute physiology and chronic health evaluation

CAPCommunity-acquired pneumonia

HAPHospital-acquired pneumonia

LPSLipopolysaccharide

MFIMean fluorescence intensity

sTREM-1Soluble triggering receptor expressed on myeloid cells-1

TREM-1Triggering receptor expressed on myeloid cells-1

VAPVentilator-associated pneumonia

Electronic supplementary materialThe online version of this article doi:10.1186-s12865-014-0063-y contains supplementary material, which is available to authorized users.

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Author: Androniki Marioli - Marina Koupetori - Maria Raftogiannis - Maria Patrani - Nikolaos Antonakos - Maria Pavlaki - Georgios A

Source: https://link.springer.com/







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