Surface display of recombinant proteins on Escherichia coli by BclA exosporium of Bacillus anthracisReport as inadecuate




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Microbial Cell Factories

, 12:81

First Online: 22 September 2013Received: 03 January 2013Accepted: 17 September 2013

Abstract

BackgroundThe anchoring motif is one of the most important aspects of cell surface display as well as efficient and stable display of target proteins. Thus, there is currently a need for the identification and isolation of novel anchoring motifs.

ResultsA system for the display of recombinant proteins on the surface of Escherichia coli was developed using the Bacillus anthracis exosporal protein BclA as a new anchoring motif. For the surface display of recombinant proteins, the BAN display platform was constructed in which a target protein is linked to the C-terminus of N-terminal domain 21 amino acids of BclA. The potential application of BAN platform for cell surface display was demonstrated with two model proteins of different size, the Bacillus sp. endoxylanase XynA and monooxygenase P450 BM3m2. Through experimental analysis including outer membrane fractionation, confocal microscopy and activity assay, it was clearly confirmed that both model proteins were successfully displayed with high activities on the E. coli cell surface.

ConclusionsThese results of this study suggest that the strategy employing the B. anthracis BclA as an anchoring motif is suitable for the display of heterologous proteins on the surface of E. coli and consequently for various biocatalytic applications as well as protein engineering.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2859-12-81 contains supplementary material, which is available to authorized users.

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Author: Tae Jung Park - Nam Su Heo - Sung Sun Yim - Jong Hyun Park - Ki Jun Jeong - Sang Yup Lee

Source: https://link.springer.com/







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