Determination of carbohydrates present in Saccharomyces cerevisiae using mid-infrared spectroscopy and partial least squares regressionReport as inadecuate




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Analytical and Bioanalytical Chemistry

, Volume 405, Issue 25, pp 8241–8250

First Online: 21 August 2013Received: 26 May 2013Revised: 24 June 2013Accepted: 09 July 2013

Abstract

A fast and simple method to control variations in carbohydrate composition of Saccharomyces cerevisiae, baker-s yeast, during fermentation was developed using mid-infrared mid-IR spectroscopy. The method allows for precise and accurate determinations with minimal or no sample preparation and reagent consumption based on mid-IR spectra and partial least squares PLS regression. The PLS models were developed employing the results from reference analysis of the yeast cells. The reference analyses quantify the amount of trehalose, glucose, glycogen, and mannan in S. cerevisiae. The selection and optimization of pretreatment steps of samples such as the disruption of the yeast cells and the hydrolysis of mannan and glycogen to obtain monosaccharides were carried out. Trehalose, glucose, and mannose were determined using high-performance liquid chromatography coupled with a refractive index detector and total carbohydrates were measured using the phenol–sulfuric method. Linear concentration range, accuracy, precision, LOD and LOQ were examined to check the reliability of the chromatographic method for each analyte.

Open image in new windowFigureComparison of workflows for carbohydrate determination in S.cerevisiae by FT-IR spectroscopy and HPLC-RI

KeywordsFT-IR spectroscopy Partial least squares regression Saccharomyces cerevisiae Carbohydrates HPLC-RI Reference analysis Electronic supplementary materialThe online version of this article doi:10.1007-s00216-013-7239-9 contains supplementary material, which is available to authorized users.

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Author: Maria R. Plata - Cosima Koch - Patrick Wechselberger - Christoph Herwig - Bernhard Lendl

Source: https://link.springer.com/



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