Photo-biohydrogen production potential of Rhodobacter capsulatus- PK from wheat strawReport as inadecuate

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Biotechnology for Biofuels

, 6:144

First Online: 07 October 2013Received: 14 April 2013Accepted: 27 September 2013


BackgroundBiotechnological exploitation of lignocellulosic biomass is promising for sustainable and environmentally sound energy provision strategy because of the abundant availability of the renewable resources. Wheat straw WS comprising of 75-80% cellulose and hemicellulose is one of widely available, inexpensive and renewable lignocellulosic biomass types. The cellulosic and hemicellulose substrate can be hydrolyzed into monomeric sugars by chemical and-or biological methods.

ResultsThis study examined comparative potential of dilute acid and pre-ammonia pretreated and enzymatically hydrolyzed wheat straw WS for hydrogen production by purple non sulfur bacterium Rhodobacter capsulatus-PK. Gas production became noticeable after 14 h of inoculation in WS pretreated with 4% H2SO4. The detoxified liquid hydrolyzate DLH after overliming attained a production level of 372 mL-H2-L after 16 h under illumination of 120-150 W-m at 30 ± 2.0°C. Whereas the non-detoxified acid pretreated hydrolyzate NDLH of WS could produce only upto 254 mL-H2-L after 21 h post inoculation. Evolution of H2 became observable just after 10 ± 2.0 h of inoculation by employing 48 h age inoculum on the WS pretreated with 30% ammonia, hydrolyzed with cellulase 80 FPU-g and β-glucosidase 220 CbU-ml at 50°C. Upto 712 ml-L of culture was measured with continuous shaking for 24 h. The 47.5% and 64.2% higher hydrogen volume than the DLH and NDLH substrates, respectively appeared as a function of significantly higher monomeric sugar contents of the enzymatically hydrolyzed substrate and lesser-zero amounts of toxic derivatives including pH reducing agents.

ConclusionPhotofermentative hydrogen production from lignocellulosic waste is a feasible approach for eco-friendly sustainable supply of bioenergy in a cost-effective way. Results of this study provide new insight for addressing biotechnological exploitation of abundantly available and low-cost cellulosic substrates.

KeywordsPNSB Hydrogen yield Cellulose Hydrogen yield and monomeric sugars Furfural and H2 Acetic acid and H2 Electronic supplementary materialThe online version of this article doi:10.1186-1754-6834-6-144 contains supplementary material, which is available to authorized users.

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Author: Saima Shahzad Mirza - Javed Iqbal Qazi - Quanbao Zhao - Shulin Chen



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