Chemical composition and antioxidant capacities of phytococktail extracts from trans-Himalayan cold desertReport as inadecuate




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BMC Complementary and Alternative Medicine

, 13:259

Basic research

Abstract

BackgroundHimalayan plants are widely used in traditional system of medicine both as prophylactics and therapeutics for high altitude maladies. Our aim was to evaluate the antioxidant capacities and bioactive compounds of methanol and n-hexane extracts of the phytococktail comprising of sea buckthorn Hippophae rhamnoides, apricot Prunus armeniaca and roseroot Rhodiola imbricata from trans-Himalaya.

MethodsThe 1,1-diphenyl-2-picrylhydrazyl DPPH, 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid diammonium salt ABTS and nitric oxide NO radical scavenging capacities and lipid peroxidation inhibition LPI property of the extracts were determined. Total antioxidant power was determined by ferric reducing-antioxidant power FRAP assay. Total polyphenol, flavonoid, flavonol, proanthocyanidin and carotenoid were also estimated for both extracts. We have identified and quantified the phyto-chemotypes present in the methanol and n-hexane extracts by hyphenated gas chromatography-mass spectrometry GC-MS technique.

ResultsAntioxidant capacity assays using DPPH, ABTS, NO, LPI and FRAP exhibited analogous results where the phytococktail showed high antioxidant action. The phytococktail was also found to possess high quantity of total polyphenol, flavonoid, flavonol and carotenoid. A significant and linear correlation was found between the antioxidant capacities and bioactive principles. A total of 32 phyto-chemotypes were identified from these extracts by GC-MS chemometric fingerprinting. Major phyto-chemotypes identified by GC-MS were glycosides, phenylpropanoids and derivatives, terpenoids, alkaloids, phytosterols, fatty acids and esters, alkaloids and derivatives, organic acid esters and aromatic ethers with positive biological and pharmacological actions.

ConclusionThe phytococktail extracts were found to contain considerable amount of diverse bioactive compounds with high antioxidant capacities. The presence of hydrophilic and lipophilic antioxidants in the phytococktail could have contributed to the higher antioxidant values. Hence, the phytococktail could be used as natural source of antioxidants to ameliorate disorders associated with oxidative stress.

KeywordsPhytococktail Sea buckthorn Apricot Roseroot Antioxidant capacity Total polyphenol content GC-MS analysis Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-13-259 contains supplementary material, which is available to authorized users.

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Author: Priyanka Dhar - Prabodh Kumar Bajpai - Amol Bapurao Tayade - Om Prakash Chaurasia - Ravi Bihari Srivastava - Shashi Bala

Source: https://link.springer.com/







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