A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of Brazilian mangrovesReport as inadecuate




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AMB Express

, 3:65

First Online: 26 October 2013Received: 17 October 2013Accepted: 21 October 2013

Abstract

The mangroves are among the most productive and biologically important environments. The possible presence of cellulolytic enzymes and microorganisms useful for biomass degradation as well as taxonomic and functional aspects of two Brazilian mangroves were evaluated using cultivation and metagenomic approaches. From a total of 296 microorganisms with visual differences in colony morphology and growth including bacteria, yeast and filamentous fungus, 179 60.5% and 117 39.5% were isolated from the Rio de Janeiro RJ and Bahia BA samples, respectively. RJ metagenome showed the higher number of microbial isolates, which is consistent with its most conserved state and higher diversity. The metagenomic sequencing data showed similar predominant bacterial phyla in the BA and RJ mangroves with an abundance of Proteobacteria 57.8% and 44.6%, Firmicutes 11% and 12.3% and Actinobacteria 8.4% and 7.5%. A higher number of enzymes involved in the degradation of polycyclic aromatic compounds were found in the BA mangrove. Specific sequences involved in the cellulolytic degradation, belonging to cellulases, hemicellulases, carbohydrate binding domains, dockerins and cohesins were identified, and it was possible to isolate cultivable fungi and bacteria related to biomass decomposition and with potential applications for the production of biofuels. These results showed that the mangroves possess all fundamental molecular tools required for building the cellulosome, which is required for the efficient degradation of cellulose material and sugar release.

KeywordsBrazilian mangrove Cellulase Metagenomics Biomass decomposition Bacterial diversity Electronic supplementary materialThe online version of this article doi:10.1186-2191-0855-3-65 contains supplementary material, which is available to authorized users.

Claudia Elizabeth Thompson, Walter Orlando Beys-da-Silva, Lucélia Santi contributed equally to this work.

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Author: Claudia Elizabeth Thompson - Walter Orlando Beys-da-Silva - Lucélia Santi - Markus Berger - Marilene Henning Vainstein -

Source: https://link.springer.com/



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