Evaluation of the content variation of anthraquinone glycosides in rhubarb by UPLC-PDAReport as inadecuate




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Chemistry Central Journal

, 7:170

Analytical Chemistry

Abstract

BackgroundRhubarb is an important Chinese medicinal herb with a long history of over 2000 years and has been commonly used as a laxative. It is the radix and rhizome of Rheum officinale Baill., R. palmatum L. and R. tanguticum Maxim, all of which are mainly distributed in a broad region in the Tibetan plateau. Anthraquinone glycosides are a series of major active ingredients found in all three species. They are key intermediates in the anthraquinone secondary metabolism and the sennnoside biosynthesis. The variation of the anthraquinone glycoside content in rhubarb in response to specific factors remains an attractive topic.

ResultsA simple and sensitive Ultra Performance Liquid Chromatography with Photo-Diode Array UPLC-PDA detector was developed for the simultaneous determination of six anthraquinone glycosides in rhubarb, i.e., aloeemodin-8-O-glucoside, rhein-8-O-glucoside, chrysophanol-1-O-glucoside, emodin-1-O-glucoside, chrysophanol-8-O-glucoside, emodin-8-O-glucoside. Twenty-seven batches from three species were submitted to the multi-component analysis. The results showed that the anthraquinone glycoside content varied significantly even within the same species. The results showed that the anthraquinone glycoside content varied significantly within the same species but not between different species. The PCA and content analysis results confirmed that the plant species has no obvious effect on the content variation. Neither was any significant correlation observed between the anthraquinone glycoside content and the geographic distribution of the rhubarb. Through correlational analysis, altitude was found to be the main factor that affects the anthraquinone glycoside content in rhubarb. Rhubarb grown at higher altitude has higher anthraquinone glycoside content.

ConclusionsThis work provides a rapid, sensitive and accurate UPLC-PDA method for the simultaneous determination of six anthraquinone glycosides in rhubarb. The anthraquinone glycoside content varied significantly within the same species. The relationship of the anthraquinone glycoside content with plant species, geographic distribution and altitude were studied using correlational analysis, principal component analysis and spatial autocorrelation analysis through SPSS and ArcGIS. Plant species and geographic distribution were found not to affect the content of the six anthraquinone glycosides in rhubarb. The variations in the anthraquinone glycoside content were primarily due to the different altitude where the plant was grown.

KeywordsRhubarb UPLC-PDA Anthraquinone glycoside Quantitative analysis Environmental factor AbbreviationsUPLCUltra performance liquid chromatography

PDAPhoto-diode array

SPSSStatistical product and service solutions

GISGeographic information system

PCAPrincipal component analysis

AE8GAloeemodin-8-O-glucoside

R8GRhein-8-O-glucoside

E1GEmodin-1-O-glucoside

C1GChrysophanol-1-O-glucoside

C8GChrysophanol-8-O-glucoside

E8GEmodin-8-O-glucoside.

Electronic supplementary materialThe online version of this article doi:10.1186-1752-153X-7-170 contains supplementary material, which is available to authorized users.

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Author: Zhe Wang - Pei Ma - Lijia Xu - Chunnian He - Yong Peng - Peigen Xiao

Source: https://link.springer.com/



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