Redescription of Onchocerca lupi Spirurida: Onchocercidae with histopathological observationsReport as inadecuate




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Parasites and Vectors

, 6:309

First Online: 27 October 2013Received: 23 September 2013Accepted: 21 October 2013

Abstract

BackgroundOnchocerca lupi is a dog parasite of increasing zoonotic concern, with new human cases diagnosed in Turkey, Tunisia, Iran, and the United States. Information about the morphology of this nematode is scant and a detailed re-description of this species is overdue. In addition, histopathological data of potential usefulness for the identification of O. lupi infections are provided.

MethodsMale and female nematodes, collected from the connective tissue of a dog, were examined using light microscopy and scanning electron microscopy SEM, and an histological evaluation was performed on biopsy samples from periocular tissues.

ResultsThe morphological identification was confirmed by molecular amplification and partial sequencing of cytochrome oxidase subunit 1 gene. This study provides the first comprehensive morphological and morphometric description of O. lupi from a dog based on light microscopy, SEM, molecular characterization, and histological observations.

ConclusionsData herein presented contribute to a better understanding of this little known parasitic zoonosis, whose impact on human and animal health is still underestimated. The presence of granulomatous reactions only around the female adult suggests that the release of microfilariae from the uterus might be the cause of the inflammatory reaction observed.

KeywordsOnchocerca lupi Vector-borne disease Scanning electron microscopy Light microscopy Histology Zoonosis Electronic supplementary materialThe online version of this article doi:10.1186-1756-3305-6-309 contains supplementary material, which is available to authorized users.

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Author: Yasen Mutafchiev - Filipe Dantas-Torres - Alessio Giannelli - Francesca Abramo - Elias Papadopoulos - Luís Cardoso - Helder

Source: https://link.springer.com/



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