Monitoring of the bacterial and fungal biodiversity and dynamics during Massa Medicata Fermentata fermentationReport as inadecuate




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Applied Microbiology and Biotechnology

, Volume 97, Issue 22, pp 9647–9655

First Online: 28 August 2013Received: 12 July 2013Revised: 07 August 2013Accepted: 10 August 2013

Abstract

The microbial community dynamics play an important role during Massa Medicata Fermentata MMF fermentation. In this study, bacterial and fungal communities were investigated based on the culture-dependent method and polymerase chain reaction-denaturing gradient gel electrophoresis analysis. Meanwhile the dynamic changes of digestive enzyme activities were also examined. Plating results showed that MMF fermentation comprised two stages: pre-fermentation stage 0–4 days was dominated by bacterial community and post-fermentation stage 5–9 days was dominated by fungal community. The amount of bacteria reached the highest copy number 1.2 × 10 CFU-g at day 2, but the fungi counts reached 6.3 × 10 CFU-g at day 9. A total of 170 isolates were closely related to genera Enterobacter, Klebsiella, Acinetobacter, Pseudomonas, Mucor, Saccharomyces, Rhodotorula, and Amylomyces. DGGE analysis showed a clear reduction of bacterial and fungal diversity during fermentation, and the dominant microbes belonged to genera Enterobacter, Pediococcus, Pseudomonas, Mucor, and Saccharomyces. Digestive enzyme assay showed filter paper activity; the activities of amylase, carboxymethyl cellulase, and lipase reached a peak at day 4; and the protease activity constantly increased until the end of the fermentation. In this study, we carried out a detailed and comprehensive analysis of microbial communities as well as four digestive enzymes- activities during MMF fermentation process. The monitoring of bacterial and fungal biodiversity and dynamics during MMF fermentation has significant potential for controlling the fermentation process.

KeywordsMassa Medicata Fermentata fermentation PCR-DGGE Digestive enzyme Bacterial community Fungal community  Download fulltext PDF



Author: Yun Xu - Yan-Bo Xie - Xin-Ran Zhang - Chen Chen - Hongyu Xiang - Qiuhong Xie

Source: https://link.springer.com/







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