Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entryReport as inadecuate




Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry - Download this document for free, or read online. Document in PDF available to download.

Virology Journal

, 10:331

Negative-strand RNA viruses

Abstract

BackgroundUsing reverse genetics, we generated a recombinant low-pathogenic LaSota strain Newcastle disease virus NDV expressing the glycoprotein GP of Ebola virus EBOV, designated rLa-EBOVGP, and evaluated its biological characteristic in vivo and in vitro.

ResultsThe introduction and expression of the EBOV GP gene did not increase the virulence of the NDV vector in poultry or mice. EBOV GP was incorporated into the particle of the vector virus and the recombinant virus rLa-EBOVGP infected cells and spread within them independently of exogenous trypsin. rLa-EBOVGP is more resistant to NDV antiserum than the vector NDV and is moderately sensitive to EBOV GP antiserum. More importantly, infection with rLa-EBOVGP was markedly inhibited by IPA3, indicating that rLa-EBOVGP uses macropinocytosis as the major internalization pathway for cell entry.

ConclusionsThe results demonstrate that EBOV GP in recombinant NDV particles functions independently to mediate the viral infection of the host cells and alters the cell-entry pathway.

KeywordsRecombinant Newcastle disease virus Ebola virus Glycoprotein Virus entry Macropinocytosis Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-10-331 contains supplementary material, which is available to authorized users.

Zhiyuan Wen, Bolin Zhao contributed equally to this work.

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Author: Zhiyuan Wen - Bolin Zhao - Kun Song - Xule Hu - Weiye Chen - Dongni Kong - Jinying Ge - Zhigao Bu

Source: https://link.springer.com/







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