A multiscale study of bacterial proliferation modes within novel E. coli@SiHIPE hybrid macrocellular living foamsReport as inadecuate




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* Corresponding author 1 UPR8641 - Centre de recherche Paul Pascal, CNRS, Université de Bordeaux 2 Collège de France, Chimie de la Matière Condensée de Paris 3 UPMC - Université Pierre et Marie Curie - Paris 6 4 LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris 5 MHN - Matériaux Hybrides et Nanomatériaux LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris

Abstract : For the first time the study at various length scales of E. coli proliferation modes within SiHIPE inorganic macrocellular foams is proposed. Both qualitatively and semi-quantitatively, the bacterial proliferation within the foam is not homogeneous and is directly linked to the SiHIPE-s macroscopic cells random distribution. When inoculated in a nutrients loaded SiHIPE, the bacterial growth is enhanced within the SiHIPE matrices compared to the surrounding LB media. The bacteria growth kinetics tends to be faster and the concentration at saturation is roughly 100% times higher. In the case of a SiHIPE host free of nutrients, the bacterial motion is occurring as an infiltration wave, the peak of this propagation wave moving at a constant speed of 88 µm h-1 , while bacterial concentrations within the SiHIPE reach levels far above the ones reached with the presence of nutrients, suggesting a real synergetic relation between the bacterial colony guests and the SiHIPE host. When a nutrients reservoir is present at the opposite position from which bacteria were inoculated, the bacterial proliferation is associated to a coalescence process between the growing colonies that were rapidly established within the first hours. When the SiHIPE is fully colonized we found out a specific distance between adjacent colonies of 5 to 15 µm in good correspondence with the repartition of the wall to wall distances of the SiHIPE-s macroscopic cells, meaning that the bacterial repartition once the colonization occurred is optimum. These results show that SiHIPE foams represent outstanding candidates for strengthened bacterial proliferation without the motion restriction imposed by conventional silica gels.





Author: Martin Depardieu - Mélanie Viaud - Axel Buguin - Jacques Livage - Clément Sanchez - Rénal Backov -

Source: https://hal.archives-ouvertes.fr/



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