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DARU Journal of Pharmaceutical Sciences

, 21:73

First Online: 20 December 2013Received: 22 June 2013Accepted: 31 August 2013

Abstract

BackgroundBerberine was encapsulated in yeast cells of Saccharomyces cerevisiae as novel carriers to be used in different food and drug industries. The microcapsules were characterized by differential scanning calorimetry DSC, fourier transform infra red spectroscopy FT-IR and fluorescence microscopy. The encapsulation factors such as plasmolysis of yeast cells which affects the % encapsulation yield were studied.

ResultsFluorescence microscopy showed the yeast cells became fluorescent after encapsulation process. DSC diagram was representing of new peak for microcapsule which was not the same as berberine and the empty yeast cells peaks, separately. FTIR spectrums of microcapsules and yeast cells were almost the same. The plasmolysed and non plasmolysed microcapsules were loaded with berberine up to about 40.2 ± 0.2% w-w.

ConclusionAnalytical methods proved that berberine was encapsulated in the yeast cells. Fluorescence microscopy and FTIR results showed the entrance of berberine inside the yeasts. DSC diagram indicated the appearance of new peak which is due to the synthesis of new product. Although plasmolysis caused changes in yeast cell structure and properties, it did not enhance berberine loading in the cells. The results confirmed that Saccharomyces cerevisiae could be an efficient and safe carrier for active materials.

KeywordsBerberine Encapsulation Saccharomyces cerevisiae yeast cells Electronic supplementary materialThe online version of this article doi:10.1186-2008-2231-21-73 contains supplementary material, which is available to authorized users.

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Author: Roshanak Salari - BiBi Sedigheh Fazly Bazzaz - Omid Rajabi - Zahra Khashyarmanesh

Source: https://link.springer.com/







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