Expression of antiapoptosis gene survivin in luteinized ovarian granulosa cells of women undergoing IVF or ICSI and embryo transfer: clinical correlationsReport as inadecuate




Expression of antiapoptosis gene survivin in luteinized ovarian granulosa cells of women undergoing IVF or ICSI and embryo transfer: clinical correlations - Download this document for free, or read online. Document in PDF available to download.

Reproductive Biology and Endocrinology

, 10:74

First Online: 07 September 2012Received: 18 July 2012Accepted: 30 August 2012

Abstract

BackgroundThe purpose of the study was to determine the incidence of survivin gene expression in human granulosa cells during ovarian stimulation in Greek women with normal FSH levels, undergoing IVF or ICSI and to discover any correlation between levels of gene expression and clinical parameters, efficacy of ovulation or outcomes of assisted reproduction.

MethodsTwenty nine women underwent ovulation induction for IVF or ICSI and ET with standard GnRH analogue-recombinant FSH protocol. Infertility causes were male and tubal factor. Cumulus–mature oocyte complexes were denuded and the granulosa cells were analyzed for each patient separately using quantitative reverse transcription polymerase chain reaction analysis for survivin gene expression with internal standard the ABL gene.

ResultsThe ABL and survivin mRNA were detected in granulosa cells in 93.1%. The expression levels of survivin were significantly lower in normal women male infertility factor compared to women with tubal infertility factor p = 0.007. There was no additional statistically significant correlation between levels of survivin expression and estradiol levels or dosage of FSH for ovulation induction or number of dominant follicles aspirated or number of retrieved oocytes or embryo grade or clinical pregnancy rates respectively.

ConclusionsHigh levels of survivin mRNA expression in luteinized granulosa cells in cases with tubal infertility seem to protect ovaries from follicular apoptosis. A subpopulation of patients with low levels of survivin mRNA in granulosa cells might benefit with ICSI treatment to bypass possible natural barriers of sperm-oocyte interactions.

KeywordsGranulosa cells Infertile patients Survivin IVF ICSI AbbreviationsIVFIn Vitro Fertilization

ICSIIntra Cytoplasmic Sperm Injection

ETEmbryo Transfer

FSHFollicle-Stimulating Hormone

rFSHrecombinant FSH

LHLuteinizing Hormone

LHRLH Receptor

GnRHGonadotropin Releasing Hormone

GnRH-aGnRH-analogue

ARTAssisted Reproductive Technology

RRMRNA Recognition Motif

RIARadio Immuno Assay

PRLProlactine

AMHAntiMüllerian Hormone

P4Micronized Progesterone

OHSSOvarian Hyperstimulation Syndrome

BMIBody Mass Index

NORNormal Ovarian Reserve

DORDiminished Ovarian Reserve

FF-MASFollicular Fluid Meiosis-Activating Sterol

LDMLanosterol 14α-Demethylase

EGFREpidermal Growth Factor Receptor

PKCProtein kinase C

CEOsCumulus Enclosed Oocytes

CMOCsCumulus-Mature Oocyte Complexes

CREBCAMP Response Element-Binding protein

CYP51Cytochrome P450 Lanosterol 14αDemethylase.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-7827-10-74 contains supplementary material, which is available to authorized users.

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Author: Michail Varras - Katerina Polonifi - Marina Mantzourani - Konstantinos Stefanidis - Zacharias Papadopoulos - Christodoulos A

Source: https://link.springer.com/



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